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The Fish Oocyte : from basic studies to biotechnological applications
Addresses the growing needs in deciphering the biological processes associated with fish reproduction, in view of the growth of aquaculture and the dwindling natural stocks of commercially important fish. It presents a comprehensive overview on egg production in fish, from the standpoint of the oocyte. With this view in mind, the book includes chapters on oocyte development (oogenesis), hormonal regulation and hormone receptors, formation of the egg envelopes, growth, accumulation of nutrients and maternal transcripts, maturation, hydration, ovulation and fertilization. A special emphasis is placed on using state-of-the-art tools including electron microscopy for discerning the ultra-structure of the follicle and genomic/proteomic tools to fully understand biological basis of fish reproduction.
RNA analysis : Methods and protocols
Explores the latest technologies for RNA analysis, covering fundamental techniques and innovative approaches that expand our understanding of RNA biology. The chapters in this book are organized into four parts: reverse transcription and RNA capture methods; high-throughput sequencing methods; RNA maturation: splicing, polyadenylation, and epitranscriptomic modifications; and RNA surveillance: nonsense-mediated mRNA decay. These chapters aim to provide detailed protocols for select techniques used by molecular and computational biologists to isolate, amplify, detect, modify, and analyze RNA to gain insight into a wide range of cellular functions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Ribosome biogenesis : Methods and protocols
This volume provides comprehensive reviews and describes the latest techniques to study eukaryotic ribosome biogenesis. For more than 50 years ribosomes are a major research topic. Our knowledge about ribosome biogenesis and function such as transcription, mRNA modification, and translation was the sine qua non for developing the powerful RNA-based vaccines against RNA-viruses causing the world-threatening Covid-19 pandemia. The chapters in this book are organized into six parts. Part One discusses a comparative survey about the unity and diversity of ribosome biogenesis in pro- and eukaryotic cells. Part Two deals with the genomic organization of eukaryotic rDNA and the role of RNA polymerase I in ribosomal RNA transcription. Part Three explores in vitro methods to study RNA polymerase I structure and its function, and Part Four analyzes the nucleo-cytoplasmic transport of assembled ribosomes and RNP complexes. Part Five covers modifications that increase the complexity of rRNAs, and Part Six provides readers with a review of eukaryotic translation and - for the first time - describes a new method to analyze translation in vitro.
Regulation of carcinogenesis, angiogenesis and metastasis by the proprotein convertases (PCs) : A new potential strategy in cancer therapy
The purpose of this book is to provide detailed and updated information on the role of these molecules in cancer. This book is the first to summarize current knowledge about the importance of protein precursors maturation by the convertases in tumor progression, angiogenesis and metastasis. In each chapter, the importance of the convertases in the activation of various cancer-related molecules including growth factors, adhesion molecules and proteases is discussed. Also this book covers the role of some convertases in the clinical setting.
Rapid Integration of Software Engineering Techniques ; Vol. 3475 : 1st International Workshop, RISE 2004, Luxembourg-Kirchberg, Luxembourg, November 26, 2004, Revised Selected Papers
This paper reports on the experiences of integrating several Software Engineering techniques by the Rapid Prototyping group of Siemens Corporate Research. This experience was gained during our recent project that involved developing a web-based, workflow-driven information system. The techniques integrated for this project included agile and iterative processes, user centered design, requirements discovery and maturation, and test-driven development. These techniques were integrated and supported by a proprietary process entitled “Siemens Rapid Prototyping” (S-RaP), a software architecture, and project management techniques. This paper will detail the specific characteristics of S-RaP, the software architecture, and the project management techniques that supported the integration of the above listed software engineering techniques. We will also report on our experience with their effectiveness and our thoughts on future enhancements in all three areas
q-Clan Geometries in Characteristic 2
This monograph offers the only comprehensive, coherent treatment of the theory - in characteristic 2 - of the so-called flock quadrangles, i.e., those generalized quadrangles (GQ) that arise from q-clans, along with their associated ovals. Special attention is given to the determination of the complete oval stabilizers of each of the ovals associated with a flock GQ. A concise but logically complete introduction to the basic ideas is given. The theory of these flock GQ has evolved over the past two decades and has reached a level of maturation that makes it possible for the first time to give a satisfactory, unified treatment of all the known examples.
Protocol for Somatic Embryogenesis in Woody Plants
These volumes provided readers extensive reviews on somatic embryogenesis of important angiosperm and gymnosperm tree species; an excellent source of information for newcomers or those already engaged in research. However, these book volumes did not cover stepwise "detailed protocols" for inducing somatic embryogenesis. This book contains 46 chapters, divided into 4 sections: A) 12 chapters on conifers, B) 14 chapters on fruits, C) 14 chapters on angiosperms, and D) 6 chapters on histology, bioencapsulation, protoplasts, cryopreservation, double staining and thin cell layer sectioning. The book provides stepwise protocols for somatic embryogenesis of a range of selected woody plants in order to assist researchers to initiate somatic embryogenic cultures without too much alterations in protocols. Each chapter provides information on initiation and maintenance of embryogenic cultures; somatic embryo development, maturation, and germination; acclimitization and field transfer of somatic seedlings. Some chapteres include applications of somatic embryogenesis cultures, e.g. protoplasts, encapsulation, cryopreservation, genetic transformation, genetic fidelity with molecular markers, and bioreactor.
Peer Review, Peer Education, and Modeling in the Practice of Clinical Ethics Consultation : The Zadeh Project
About the Zadeh Project demonstrates and explores a core question in clinical ethics: how can ethics consultants be accountable in the face of a robust plurality of ethical standpoints, especially those that underwrite practices and methods for doing ethics consultation as well as those viewpoints and values encountered in daily clinical ethics practice? Underscoring this question is the recognition that the field of clinical ethics consultation has arrived at a crucial point in its maturation.
Neonatal Cranial Ultrasonography
It provides important information on brain maturation in the (preterm) neonate and enables the detection of frequently occurring brain anomalies in both preterm and full term neonates. Furthermore, it can be repeated as often as is necessary. In this edition of Neonatal Cranial Ultrasonography, the focus is on the basics of the technique, from patient preparation through to screening strategies and the classification of abnormalities. Many new ultrasound images have been included to reflect the improvements in image quality since the first edition. Essential information is provided about both the procedure itself and the normal ultrasound anatomy. Standard technique using the anterior fontanelle as the acoustic window is described and illustrated, but emphasis is also placed on the value of supplementary acoustic windows.
Mechanisms of mineralization of vertebrate skeletal and dental tissues
Presents a multi-disciplinary approach to understanding mechanisms regulating the formation of mineral in vertebrate skeletal and dental tissues. The focus of the book is directed toward the mineralization process, an evolutionarily conserved system in which cells synthesize a complex and unique extracellular matrix into which mineral is deposited. Regulatory control is viewed though lenses that emphasize the genetic, physical-chemical, biochemical, structural, cellular and extracellular aspects of the mineralization process as they relate to crystal nucleation, growth and maturation. Throughout the book, defects in regulation at the genetic and transcriptional levels are linked to the numerous clinical problems associated with the mineralization of bone, cartilage, tendon, tooth, and soft tissues.
Immune receptors : Methods and protocols
Explores immune cell receptors that are used in the detection of microbes, either by binding directly to non-self molecules or through indirectly sensing microbe-associated cellular disturbances. The covers methods for studying receptor-ligand interactions at both molecular and cellular levels; methods to create and characterize novel antibody reagents; and methods to characterize the molecular processes that lead to adaptive receptor maturation. This book also contains chapters that look at high-throughput strategies that describe the diversity of immune receptors and cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Fuzzy Systems Engineering : Theory and Practice
This book is devoted to reporting innovative and significant progress in fuzzy system engineering. Given the maturation of fuzzy logic, this book is dedicated to exploring the recent breakthroughs in fuzziness and soft computing in favour of intelligent system engineering. This monograph presents novel developments of the fuzzy theory as well as interesting applications of the fuzzy logic exploiting the theory to engineer intelligent systems.
Engineered Bamboo Structures
Bamboo is in the spotlight as a potential building material in the current pursuit of a CO2-neutral society, due to its rapid maturation and excellent mechanical properties. Despite the growing interest in bamboo in academia and society, there is a lack of systematic understanding of the fabrication, design and construction processes using bamboo as a modern industrial material. Describes a new category of structural systems constructed with engineered bamboo. It gives a definition of engineered bamboo (glubam) in an analogy with steel structures and wood structures. Structural systems and components have been designed using glubam; then industrialized production processes of glubam are described.
Damage and Cracking of Concrete Structures : From Theory to Practice
Understanding and managing damage and cracking in concrete is essential to ensuring the integrity and durability of civil engineering structures. This book also addresses many situations, such as monotonic or cyclic behavior, seismic responses, a description of fast dynamic situations and effects due to the maturation of concrete at an early age in massive structures. Numerous detailed exercises are provided to help students to understand modeling and calculation techniques.
