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Liver carcinogenesis : Methods and protocols
Discusses the latest advancements in modern methodologies used to study liver carcinogenesis. The first half of this book describes pertinent preclinical models of hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA), established either through orthotopic induction of ectopic implantation. The second half of this book covers a diverse array of techniques applied to characterize the biochemical and cellular composition of hepatic malignancies that operate at the single-cell and histological levels. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Lifestyle nursing
Examines the concepts of lifestyle medicine and nursing practice, it is specifically designed to help nurses introduce the concepts of lifestyle medicine to readers while also encouraging them to focus on their own wellness. This book features nutritional guidelines and supplemental materials operationalizing this basic nutrition knowledge into personal and patient wellness. It addresses evidence-based findings of chronic diseases including heart diseases and stroke, type 2 diabetes, and cancers, which can often be prevented by lifestyle interventions.
Lactic acid bacteria : Methods and protocols
This updated volume presents experimentation-based approaches to lactic acid bacteria (LAB) research. Split into three parts, the book explores techniques for analyzing lactic acid bacteria metabolism and characteristics, applications for food-related industries, such as yogurt production, beer, and wine making, and functions of LAB in human health. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topic, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Clinical applications of mass spectrometry in drug analysis : Methods and protocols
This fully updated volume describes methods and protocols for a number of drugs and toxins in a stepwise manner. Exploring the versatility and flexibility of mass spectrometry, the book covers the advantages of this technology, which typically include elimination of the need for special reagents such as antibodies, increased sensitivity and specificity, and multi-component analysis enabling the screening of tens to hundreds of compounds in a single assay run. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls.
Cleaning validation : Practical compliance approaches for pharmaceutical manufacturing
Timely coverage of cleaning validation for the pharmaceutical industry, a dynamic area in terms of health-based limits. Encourages pharmaceutical manufacturers, and particularly upper management, to meet the challenges of the science-based and riskbased approaches to cleaning validation. Draws on the author’s vast experience in the field of cleaning validation and hazardous materials. Discusses EMA vs. ISPE on Cleaning Limits and revised Risk-MaPP for highly hazardous products in shared facilities. A diverse list of topics from protocol limits for yeasts and molds to cleaning validation for homeopathic drug products.
Cilia : Methods and protocols
Covers the latest advancements in the study of ciliary complexity. Protocols cover genomic, proteomic, imaging, and functional analysis of different ciliated tissues and their wide applicability in cilia biology. Chapters in this book primarily focus on methods to study multiciliated cells, and discuss topics such as SARS-CoV-2 infections of human primary nasal multiciliated epithelial cells; expansion microscopy of ciliary proteins; live-imaging centriole amplification in mouse brain multiciliated cells; biophysical properties of cilia motility; and mucociliary transport device construction. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Chitosan-based nanocomposite materials : fabrication, characterization and biomedical applications
Discovery in the use of chitosan-based nanocomposites in biomedical applications, including the scope to which these novel materials have been incorporated by the community. It provides an exceptional insight into the strategies for the synthesis and chemical modifications of chitosan, characterization techniques, their use as anticancer agents, antimicrobial, antiviral, and antifungal agents, their role in the biomedical field, and applications in drug delivery, gene therapy, dentistry, orthopedics, etc. This book will also emphasize the challenges with previous signs of progress and way for further research, details relating to the current pioneering technology, and future perspectives with a multidisciplinary approach. Furthermore, it presents up-to-date information on the economics, toxicity, and regulations related to these novel materials.
Chitosan : properties and applications in bioengineering
Presents a review of chitosan, including information regarding its structure, main properties, available sources, extraction, and processing techniques. It presents the different applications of chitosan as a biomaterial in fields such as medicine and the agricultural, cosmetology, pharmaceutical, and food industries to have readers obtain a more technical vision of the current use of this biopolymer.
Chitin and Chitosan : Properties and Applications
Chitin and Chitosan: Properties and Applications presents a comprehensive review of the isolation, properties and applications of chitin and chitosan. These promising biomaterials have the potential to be broadly applied and there is a growing market for these biopolymers in areas such as medical and pharmaceutical, packaging, agricultural, textile, cosmetics, nanoparticles and more.
Chemometrics and Cheminformatics in Aquatic Toxicology
Chemometrics and Cheminformatics in Aquatic Toxicology delivers an exploration of the existing and emerging problems of contamination of the aquatic environment through various metal and organic pollutants, including industrial chemicals, pharmaceuticals, cosmetics, biocides, nanomaterials, pesticides, surfactants, dyes, and more. The book discusses different chemometric and cheminformatic tools for non-experts and their application to the analysis and modeling of toxicity data of chemicals to various aquatic organisms.
Chemical principles of nanoengineering
Nanomaterials, which possess at least one dimension lower than 100 nanometers, are increasingly at the forefront of technological and chemical innovation. The properties of these uniquely minute materials give them distinctive applications across a huge range of industries and research fields. It is therefore critical that the next generation of engineers and materials scientists understand these materials, their chemical properties, and how they form bonds
Chemical and functional properties of food components
Described the contents of food raw materials and products, the chemistry/biochemistry of food components, as well as the changes occurring during post-harvest storage and processing affecting the quality of foods. Discusses the role of chemical compounds in the structure of raw materials and the formation of different attributes of food quality, including nutritional value, safety, and sensory properties. It contains four new chapters: “Non-Protein Nitrogenous Compounds”; “Prooxidants and Antioxidants in Food”; “Non-Nutritive Bioactive Compounds in Food of Plant Origin”; and “Analytical Methods Used for Assessing the Quality of Food Products.”
Cell-secreted vesicles : Methods and protocols
Pesents hands-on technological protocols used to target an array of cell-secreted extracellular vesicles (EVs) in a variety of biological systems. Beginning with methods for EV purification and analysis, the book continues with sections on the study of EV functions as well as specific systems and models allowing for the study of EVs of different origin. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cell cycle control : Methods and protocols
Collects techniques to study the highly regulated cell cycle process. Beginning with chapters investigating these processes and assessing how cells respond when these complicated pathways are simplified by using synthetic biology and in vitro reconstitutions, the book continues by exploring how cells sense and respond to environmental conditions, different model systems and cellular types used to visualize cellular architecture during cell division, as well as innovative single cell microscopy techniques to highlight the heterogeneity of the cell population with respect to cell cycle progression. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Catalytic role of amino acids in organic reactions
Asymmetric organometallic and organocatalytic processes have attracted great interest. Asymmetric synthesis using both natural and unnatural amino acids has been tremendously important from synthetic as well as industrial viewpoints, and numerous new methodologies have been developed in the last decades. Herein we provide an overview of old and very recent (since 1900 till now) advances and applications in the area of heterogeneous catalysis, homogeneous catalysis, electrocatalysis, photocatalysis, organocatalysis, thermal catalysis using amino acids [proline, glycine, alanine, valine, serine, threonine, cysteine, methionine, asparagine, glutamine, lysine, arginine, histidine, aspartate, glutamate, phenylalanine, and tryptophan], (supported or unsupported), an amino acid containing materials or amino acids derivatives as an essential component of catalysts, this book highlights the most important and recent developments to immobilize or support amino acids on various support materials. This book is suitable as supplementary reading for courses targeting the design, synthesis, and application of chiral catalysts, asymmetric catalysis, and sustainable production.
Cardiac reconstructions with allograft tissues
Cryopreserved allograft tissues are now standard materials for the reconstructive cardiac surgeon. Since publication of the first edition ("Cardiac Reconstructions with Allograft Valves") in 1989, the field has progressed dramatically with increased clinical use of cardiovascular allograft tissues, with the development of new surgical techniques, and with advances in the understanding of the fundamentals of valve transplantation biology and cryopreservation. As a result, over two-thirds of the present volume represents new material. Fifty-six authors bring their expertise to thirteen comprehensive, lavishly illustrated sections which discuss the principles of the use of homograft valves, major clinical series of homograft valves for both left and right ventricular outflow tracts, cryopreserved allograft tissue for cardiac reconstruction, cell biology of heart valve leaflets, cryobiology of heart valve preservation, morphological, biochemical, and explant pathology studies of allograft heart valves, allograft valve banking, as well as detailed explanation of surgical techniques for valve and root methods for left and right ventricular outflow tract reconstructions, the Ross operation and variants, and complex reconstructions. A final section presents potential future directions for the field. Over 400 illustrations, created expressly for this book, depict the surgical techniques from the perspective of the surgeon standing at the operating table.
Cardiac gene therapy : Methods and protocols
Discussions on topics such as gene suppression, editing, and reprogramming; cardiac gene therapy vectors and promoters; cardiac gene delivery methods; pulmonary hypertension; and patient screening and measuring the efficacy of cardiac gene therapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Carbon-based nanocarriers for drug delivery
Carbon-Based Nanocarriers for Drug Delivery enlists the latitudes and advancements in the synthesis processes, functionalization, and applications of carbon-based nanomaterials (CBNs) in targeted drug delivery systems (DDSs). It covers the applicability and suitability of CBNs as nanocarriers for efficient drug delivery application via elucidating the recent advancements in CBNs, their functionalized and innovative derivatives, and the relevant case studies. The book explores the necessity, efficacy, toxicological aspects, and challenges for the application of CBN in targeted DDSs.
Cancer immunotherapy : Methods and protocols
Details multiple areas of new and emerging methods to develop the next generation of immunotherapy treatments. Chapters guide readers through analysis and characterisation of the interactions between tumour and immune cells, and cell engineering tools for cancer treatment, to provide a unique and compelling set of techniques instrumental to work with, and engineer, immune cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Borrelia burgdorferi : Methods and protocols
Covers the latest advancements and techniques used to understand the fastidious bacterium, Borrelia burgdorferi, and its significance in infectious disorders by combining both conventional and cutting-edge approaches. This book covers diverse topics, including direct detection, diagnostic methods, immune response analysis, alternative model systems, advanced proteomics, social media analysis, and clinical research. It also discusses unconventional wet lab research such as content analysis, the use of ChatGPT, clinical algorithms for chronic Lyme, establishment of a pregnancy Lyme disease biobank, and investigates Lyme in pregnant women. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
