Cell-secreted vesicles : Methods and protocols

Cell-secreted vesicles : Methods and protocols

Author
Seppo Vainio
Publication Year
2023
Publisher
Springer
Language
English
Document Type
Book
Faculty / Subject Heading
Medical Science

Pesents hands-on technological protocols used to target an array of cell-secreted extracellular vesicles (EVs) in a variety of biological systems. Beginning with methods for EV purification and analysis, the book continues with sections on the study of EV functions as well as specific systems and models allowing for the study of EVs of different origin. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Keywords: Biotechnology / Biomechanics / Cell biology / Extracellular vesicles (EVs) / Cellular regulatory signaling system / Genetic engineering / Therapeutic development / Cell organelles - Laboratory manuals / Extracellular matrix proteins - Laboratory manuals / Extracellular vesicles - Metabolism / Extracellular matrix proteins