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Natural Products : Drug Discovery and Therapeutic Medicine
Although the natural product drug discovery programs of the large drug companies are now equaled by programs for the high throughput screening of synthetic compounds generated through combinatorial chemistry, natural compounds still hold great promise to overcome such problems as antibiotic resistance, the emergence of new diseases, the failure to conquer old diseases, and the toxicity of some contemporary medical products. In Natural Products: Drug Discovery and Therapeutic Medicine, a panel of recognized experts and leaders in the field discuss the past successes of natural products as medicines and review future possibilities arising from both conventional and new technologies. High-performance liquid chromatography profiling, combinatorial synthesis, genomics, proteomics, DNA shuffling, bioinformatics, and genetic manipulation all now make it possible to rapidly evaluate the activities of extracts as well as purified components derived from microbes, plants, and marine organisms. The authors apply these methods to new natural product drug discovery, to accessing microbial diversity, to investigating specific groups of products (Chinese herbal drugs, antitumor drugs from microbes and plants, terpenoids, and arsenic compounds), and to exploiting specific sources (the sea, rainforest, and endophytes). These new opportunities show how research and development trends in the pharmaceutical industry can advance to include both synthetic compounds and natural products, and how this paradigm shift can be more productive and efficacious.
Metabolomics : The frontier of systems biology
Metabolism is the sum of the chemical reactions in cells that produce life-sustaining chemical energy and metabolites. In the post-genome era, metabolism has taken on new significance for biological scientists: metabolites are the chemical basis of phenotypes that are final expressions of genomic information. This book covers research on metabolomics, ranging from the development of specialized chemical analytical techniques to the construction of databases and methods for metabolic simulation. The authors have been directly involved in the development of all the subject areas, including capillary electrophoresis, liquid chromatography, mass spectrometry, metabolic databases, and metabolic simulation. Breakthrough achievements and the future of metabolome studies are described, making this book a valuable source for researchers in metabolomics in diverse fields, such as plant, animal, cellular, microbial, pharmaceutical, medical, and genetic sciences.
Fundamentals of analytical toxicology : Clinical and forensic ; 2nd ed.
An integrated introduction to the analysis of drugs, poisons, and other foreign compounds in biological and related specimens. Assuming only basic knowledge of analytical chemistry, this invaluable guide helps trainee analytical toxicologists understand the principles and practical skills involved in detecting, identifying, and measuring a broad range of compounds in various biological samples. Clear, easy-to-read chapters provide detailed information on topics including sample collection and preparation, spectrophotometric and luminescence techniques, liquid and gas-liquid chromatography, and mass spectrometry including hyphenated techniques. Focuses on analytical, statistical, and pharmacokinetic principles / Describes basic methodology, including colour tests and immunoassay and enzyme-based assays / Outlines laboratory operations, such as method validation, quality assessment, staff training, and laboratory accreditation / Follows IUPAC nomenclature for chemical names and recommended International Non-proprietary Name (rINN) for drugs and pesticides / Includes discussion of 'designer drugs' (novel pharmaceutical substances NPS)
Clinical applications of mass spectrometry in drug analysis : Methods and protocols
This fully updated volume describes methods and protocols for a number of drugs and toxins in a stepwise manner. Exploring the versatility and flexibility of mass spectrometry, the book covers the advantages of this technology, which typically include elimination of the need for special reagents such as antibodies, increased sensitivity and specificity, and multi-component analysis enabling the screening of tens to hundreds of compounds in a single assay run. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls.
Carbohydrate-protein interactions : Methods and protocols
Provides new and updated tools for studying protein-carbohydrate interactions ranging from traditional biochemical methods to state-of-the-art techniques. This book focuses on four different research themes detailing methods for screening and quantifying CAZyme activity, investigating the interactions between proteins, carbohydrate ligands, methods for the visualization of carbohydrates, protein-carbohydrate complexes, structural and “omic” approaches for studying systems of CAZymes.
Cancer cell biology : methods and protocols
Provides detailed methods on the mechanisms of underlying cancer cell biology. Chapters guide readers through techniques for culturing cancer cell lines, xenografts, cryopreservation of tumor cells, analyzing the co-culture of breast cancer cells, protein secretion by ELISA, flow cytometry-based, multi-parametric immunofluorescence analysis, protein expression by western blot, analysis of surface protein levels, protein recycling by biotinylation assay, and proteomics analysis by liquid chromatography-mass spectrometry.
