Cancer cell biology : methods and protocols
- Author
- Sherri L. Christian
- Publication Year
- 2022
- Publisher
- Springer
- Language
- English
- Document Type
- Book
- Faculty / Subject Heading
- Medical Science
- Download Book Read book
Provides detailed methods on the mechanisms of underlying cancer cell biology. Chapters guide readers through techniques for culturing cancer cell lines, xenografts, cryopreservation of tumor cells, analyzing the co-culture of breast cancer cells, protein secretion by ELISA, flow cytometry-based, multi-parametric immunofluorescence analysis, protein expression by western blot, analysis of surface protein levels, protein recycling by biotinylation assay, and proteomics analysis by liquid chromatography-mass spectrometry.
Keywords: Biobanking / Chemotaxis assay / Flow-cytometry / CRISPR/Cas / Immunofluorescence / Cancer research / Cell biology / Cancer cells - Laboratory manuals / Cytology - Laboratory manuals / Cancer - Genetic aspects / Neoplasms
