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Adult stem cells : Methods and protocols
Expands on the previous edition with updated chapters covering a broader range of tissues and techniques pertaining to stem cell technologies. The chapters also cover topics such as the generation of iPSC-derived cells unique to the individual human genome addressing the possibility of more personalized clinical applications to an individual with a specific degenerative disease; and the use of nanoparticles such as 3D scaffolds and biomaterials as a means of improving stem cell viability after transplantation in the host tissue. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Adipose-derived stem cells : Methods and protocols
Discusses of the latest technology and advancements in the field of human-derived ASC and SVF. The chapters in this book are organized into four parts. Part One focuses on human ASC’s isolation, characterization, and differentiation. Part Two describes the isolation and characterization of ASC and SVF from canine, feline, and murine tissues. Part Three looks at hydrogels, scaffolds, and microphysiological systems, and Part Four talks about the new assays and applications using ASC. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
A Field Guide to Algebra
Focuses on the structure of fields and is intended for a second course in abstract algebra. Besides providing proofs of the transcendance of pi and e, the book includes material on differential Galois groups and a proof of Hilbert's irreducibility theorem. The reader will hear about equations, both polynomial and differential, and about the algebraic structure of their solutions. In explaining these concepts, the author also provides comments on their historical development and leads the reader along many interesting paths. In addition, there are theorems from analysis: as stated before, the transcendence of the numbers pi and e, the fact that the complex numbers form an algebraically closed field, and also Puiseux's theorem that shows how one can parametrize the roots of polynomial equations, the coefficients of which are allowed to vary. There are exercises at the end of each chapter, varying in degree from easy to difficult. To make the book more lively, the author has incorporated pictures from the history of mathematics, including scans of mathematical stamps and pictures of mathematicians.
3D cell culture : Methods and protocols
Expands on the previous edition with discussions about the latest organoid models developed for many more organs; new hydrogels and devices for 3D culture; and the organoid systems that have been improved by incorporating more components of tissue microenvironments in the in vitro culture. The chapters in this book are organized into five parts and cover topics such as biofabrication, organoids, microfluidic systems, bioprinting, and image analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
