Adipose-derived stem cells : Methods and protocols
- Author
- Jeffrey Gimble, Bruce Bunnell, Trivia Frazier and Cecilia Sanchez
- Publication Year
- 2024
- Publisher
- Springer
- Language
- English
- Document Type
- Book
- Faculty / Subject Heading
- Medical Science
- Download Book Read book
Discusses of the latest technology and advancements in the field of human-derived ASC and SVF. The chapters in this book are organized into four parts. Part One focuses on human ASC’s isolation, characterization, and differentiation. Part Two describes the isolation and characterization of ASC and SVF from canine, feline, and murine tissues. Part Three looks at hydrogels, scaffolds, and microphysiological systems, and Part Four talks about the new assays and applications using ASC. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Keywords: Cell isolation / Extracellular matrices / Adipose depots / Multipotent stromal cell isolation / Soft tissue reconstruction / Biomedical and life sciences / Cell biology / Stem cells / Adipose tissues / Laboratory manuals / Cell culture / Fat cells
