3D cell culture : Methods and protocols
- Author
- Zuzana Sumbalova Koledova
- Publication Year
- 2024
- Publisher
- Springer
- Language
- English
- Document Type
- Book
- Faculty / Subject Heading
- Medical Science
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Expands on the previous edition with discussions about the latest organoid models developed for many more organs; new hydrogels and devices for 3D culture; and the organoid systems that have been improved by incorporating more components of tissue microenvironments in the in vitro culture. The chapters in this book are organized into five parts and cover topics such as biofabrication, organoids, microfluidic systems, bioprinting, and image analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Keywords: Decellularized scaffolds / Peptide hydrogels / Micropatterning / Phenotypic evaluation / Bone tissue modeling / Biofabrication / Bioprinting / Organoids / Biological techniques / Biomedical and life sciences / Cell biology