3D cell culture : Methods and protocols

3D cell culture : Methods and protocols

Author
Zuzana Sumbalova Koledova
Publication Year
2024
Publisher
Springer
Language
English
Document Type
Book
Faculty / Subject Heading
Medical Science

Expands on the previous edition with discussions about the latest organoid models developed for many more organs; new hydrogels and devices for 3D culture; and the organoid systems that have been improved by incorporating more components of tissue microenvironments in the in vitro culture. The chapters in this book are organized into five parts and cover topics such as biofabrication, organoids, microfluidic systems, bioprinting, and image analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Keywords: Decellularized scaffolds / Peptide hydrogels / Micropatterning / Phenotypic evaluation / Bone tissue modeling / Biofabrication / Bioprinting / Organoids / Biological techniques / Biomedical and life sciences / Cell biology