Baculovirus : Methods and protocols
- Author
- Manon M.J. Cox
- Publication Year
- 2024
- Publisher
- Springer
- Language
- English
- Document Type
- Book
- Faculty / Subject Heading
- Medical Science
- Download Book Read book
Provides up-to-date guidance on techniques used to work with baculoviruses and insect cells. The book provides basic methods to create recombinant baculoviruses, to improve productivity, to produce a variety of products, to purify products, to quantify baculovirus stocks or to quantify product produced, and it concludes with alternative uses of either baculovirus or insect cells as tools. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Keywords: Baculovirus expression vector system (BEVS) / Insect cell culture / Baculoviral stocks / Gene therapy vectors / Recombinant viruses / Vaccines / Virology / Biomedicine
