Bacterial secretion systems : Methods and protocols
- Author
- Laure Journet and Eric Cascales
- Publication Year
- 2024
- Publisher
- Springer
- Language
- English
- Document Type
- Book
- Faculty / Subject Heading
- Medical Science
- Download Book Read book
Cover techniques used to study secretion systems. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors as well as using reporters to follow effector transport. Written in the highly
Keywords: Microbiology / Cell fractionation / Lipoprotein localisation / Fluorescence microscopy / Isopycnic density gradients / Proteolysis / Interactions protéine-protéine - Recherche - Méthodologie / Microbiologie / Protein-protein interactions - Research - Methodology / Secretion / Sécrétion
