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Microfluidics diagnostics : Methods and protocols
Explores a wide range of microfluidic-based approaches that exploit the unique features of microfluidic devices, which hold significant potential in the field of diagnosis. Beginning with a section on microchips for sample preparation, including cell-free DNA, exosomes, and cells, the book continues by covering protein marker analysis and detection, single-cell analysis, analysis of bacteria and viruses, as well as human cell-based culture and analysis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Autoimmunity : Methods and protocols
Brings together a comprehensive and up-to-date collection of protocols that reflect the diverse experimental strategies. Chapters detail T-cell, macrophage characterization, neutrophil functional assays, organoid culture methods, spatial transcriptomics, RNA FISH, microRNA profiling, and ribosome profiling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
