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Double-stranded RNA : Methods and protocols

Examines classical and cutting-edge methods involving double-stranded RNA (dsRNA), specifically regarding isolation, visualization, characterization, production, and application. Many protocols, such as co-immunoprecipitation-based isolation of double-stranded RNA-associated protein complexes, identification of mycoviruses by dsRNA extraction, application of dsRNA for fungi disease management (Sclerotinia sclerotiorum and Botrytis cin), and production of double-stranded RNA in plants by plant viral vectors for gene silencing, can also be easily adapted for identification of viruses from other organisms, control of other pathogens, and fundamental research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips for troubleshooting and avoiding known pitfalls.

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DNA barcoding : Methods and protocols

Covers the latest advancements and methods used to study DNA barcoding. The chapters in this book are organized into six parts: theory, DNA manipulation, specialized barcoding methods, analysis of DNA barcodes, completing a successful DNA barcode project, and barcoding as citizen science. Part One looks at several theoretical issues in DNA barcoding concerned with DNA barcodes that can and should be used. Part Two discusses protocols for processing samples into DNA barcodes, including DNA isolation, sequencing and managing samples, and the resulting DNA barcodes. Part Three explores the next generation of DNA barcoding such as FRET-based detection, dual nanopore detection, skimming, and closed-tube methods. Part Four addresses programs and websites that handle DNA barcoding data, and includes character-based approaches, barcode gap methods, integrated approaches, and Barcode of Life Database

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DNA and RNA origami : methods and protocols

Detials diverse methodological approaches on the assembly and applications of DNA origami assemblies. Chapters guide readers through different synthetic and computational methods, isolation and structural characterization of 2D and 3D DNA origami nanoarchitectures, nanophotonics, drug delivery, biophysics, and synthetic biology.Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

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Directions in strong motion instrumentation ; Proceedings of the NATO SFP Workshop on Future Directions in Instrumentation for Strong Motion and Engineering Seismology, Kusadasi, Izmir, May 17-21, 2004

This book, which contains papers by invited authorities, represents a unique interaction between seismologists and earthquake engineers who examine issues of mutual concern in an overlapping area of major interest. The papers have been grouped around three major areas. -Seismic Hazard and Extreme Motions -Engineering Uses of Strong Motion Seismograms -Arrays and Observations.

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Diffusion in Condensed Matter : Methods, Materials, Models

Diffusion as the process of particle transport due to stochastic movement is a phenomenon of crucial relevance for a large variety of processes and materials. This comprehensive, handbook-style survey of diffusion in condensed matter gives detailed insight into diffusion as the process of particle transport due to stochastic movement. Leading experts in the field describe in 23 chapters the different aspects of diffusion, covering microscopic and macroscopic experimental techniques and exemplary results for various classes of solids, liquids and interfaces as well as several theoretical concepts and models. Students and scientists in physics, chemistry, materials science, and biology will benefit from this detailed compilation.

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Design of Very High-Frequency Multirate Switched-Capacitor Circuits : Extending the Boundaries of CMOS Analog Front-End Filtering

Presents the theory and the corresponding CMOS implementation of the novel multirate sampled-data analog interpolation technique. In order to tackle physical IC imperfections at very high frequency, this work discusses the circuit design and layout techniques for high-speed Switched-Capacitor (SC) circuits.

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Design of structural elements : Concrete, steelwork, masonry and timber designs to Eurocodes

Topics include the philosophy of design, sustainable development, basic structural concepts, and material properties. After an overview of structural design, the book contains self-contained chapters with numerous diagrams and worked examples on design in reinforced concrete, structural steelwork and steel/concrete composites, masonry and timber based on EN 1990-1997. Elements considered cover reinforced concrete and composite floors, isolated foundation, cantilever retaining wall, load-bearing and panel walls, stud wall and connections.

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Demystifying Internet of Things Security : Successful IoT Device/Edge and Platform Security Deployment

The IoT presents unique challenges in implementing security and Intel has both CPU and Isolated Security Engine capabilities to simplify it. This book explores the challenges to secure these devices to make them immune to different threats originating from within and outside the network. The requirements and robustness rules to protect the assets vary greatly and there is no single blanket solution approach to implement security.

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Deep Data Analytics for New Product Development

The benefits of reading this book are twofold. The first is an understanding of the stages of a new product development process from ideation through launching and tracking, each supported by information about customers. The second benefit is an understanding of the deep data analytics for extracting that information from data. These analytics, drawn from the statistics, econometrics, market research, and machine learning spaces, are developed in detail and illustrated at each stage of the process with simulated data. The stages of new product development and the supporting deep data analytics at each stage are not presented in isolation of each other, but are presented as a synergistic whole.

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Data-Driven Fault Detection and Reasoning for Industrial Monitoring

Assesses the potential of data-driven methods in industrial process monitoring engineering. The process modeling, fault detection, classification, isolation, and reasoning are studied in detail. These methods can be used to improve the safety and reliability of industrial processes. Fault diagnosis, including fault detection and reasoning, has attracted engineers and scientists from various fields such as control, machinery, mathematics, and automation engineering. Combining the diagnosis algorithms and application cases, this book establishes a basic framework for this topic and implements various statistical analysis methods for process monitoring.

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Curves and Surfaces for Computer Graphics

Computer graphics is important in many areas including engineering design, architecture, education, and computer art and animation. This book examines a wide array of current methods used in creating real-looking objects in the computer, one of the main aims of computer graphics.

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CSR and Codes of Business Ethics in the USA, Austria (EU) and China and their Enforcement in International Supply Chain Arbitrations

Analyzes the implementation of CSR reporting and codes of business conduct and ethics in the legal systems of the USA, Austria and China and their enforcement in international supply chain arbitrations. demonstrates that long-term profit maximization is increasingly intertwined with corporate ethics and CSR policies. In order to prevent window-dressing and greenwashing, certain control mechanisms and legal standards are required along the entire supply chain.

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Crime and safety in the rural : Lessons from research

This book highlights 20 reasons why crime and safety in rural areas is a topic of relevance. We attempt to untangle currently simplistic views of the rural by discussing a number of facets of the countryside as both safe and criminogenic, and more importantly, a hybrid place worth to be examined in its own right. We adopt the notion of a rural-urban continuum that captures the nuances of places of varied nature, spanning from remote and desolate spaces to accessible and connected environments of the urban fringe. Areas on the rural-urban continuum may be in constant transformation given local and global influences, which imposes challenges for policing and long-term social sustainability.

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Creep Mechanics ; 2nd ed.

The monograph offers an overview of other experimental investigations in creep mechanics. Rules for specifying irreducible sets of tensor invariants, scalar coefficients in constitutive and evolutional equations, and tensorial interpolation methods are also explained.

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CP Violation in Particle, Nuclear and Astrophysics

This book provides a collection of up-to-date lectures on the physics of CP violation. As such it covers all relevant modern fields of elementary particle, nuclear and astrophysics. Special attention is paid to the neutral meson systems and the recent confirmation of CP violation in the B meson system. The theory and the novel methods needed for these experiments are given in detail. The classical and ongoing searches for the electric dipole moment of the neutron and other null tests of time-reversal symmetry are included. An elementary introduction is given to the astrophysical implications of CP violation, to tackle the puzzle of matter--antimatter asymmetry in our Universe. The aim of the book is to present recent achievements and discuss future developments in a way accessible to both postgraduate students and nonspecialist researchers. For the experienced researcher, the book will serve as a modern source of reference on this topic.

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Covid-19 tracking application with user helping features

The era of mobile technology opens the windows to the mobile apps. The websites are vanishing and the mobile phones are emerging. In light of the development of applications on mobile and their widespread spread, especially Service (SOA) ones. It’s the time to change from conventional websites to apps, which has become the part of our daily routine. We are introducing “Covid 19 Tracker” “Covid 19 Tracker” is an interactive App used to assist the Ministry of Health to provide protection and health care for citizens and residents referred to domestic isolation or quarantine; to ensure their safety and enhance their recovery procedures.

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Cooperative Bug Isolation : Winning Thesis of the 2005 ACM Doctoral Dissertation Competition

Efforts to understand and predict the behavior of software date back to the earliest days of computer programming,over half a century ago. In the intervening decades, the need for effective methods of understanding software has only increased; so- ware has spread to become the underpinning of much of modern society, and the potentially disastrous consequences of broken or poorly understood software have become all too apparent.

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Continuous-Time Signals

The book systematically covers major principle foundations of the signals theory. The representation of signals in the frequency domain (by Fourier transform) is considered with strong emphasis on how the spectral density of a single waveform becomes that of its burst and then the spectrum of its train. Different kinds of amplitude and angular modulations are analyzed noticing a consistency between the spectra of modulating and modulated signals. The energy and power presentation of signals is given along with their correlation properties. Finally, presenting the bandlimited and analytic signals, the book elucidates the methods of their description, transformation (by Hilbert transform), and sampling.

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Construction and analysis of safe, secure, and interoperable smart devices ; Vol. 3362 : International workshop, CASSIS 2004, Marseille, France, March 10-14, 2004, Revised Selected Papers

History based access control and secure information flow / The spec# programming system / Mastering test generation from smart card software formal models / A mechanism for secure, fine-grained dynamic provisioning of applications on small devices / A type system for checking applet isolation in java card / Verification of safety properties in the presence of transactions / Modelling mobility aspects of security policies / Smart devices for next generation mobile services / A flexible framework for the estimation of coverage metrics in explicit state software model checking / Combining several paradigms for circuit validation and verification / Smart card research perspectives

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Consciousness : From Perception to Reflection in the History of Philosophy

Consciousness: From Perception to Reflection in the History of Philosophy shows that the concept of consciousness was explicated relatively late in the tradition, but that its central features, such as reflexivity, subjectivity and aboutness, attained avid interest very early in philosophical debates. This book reveals how these features have been related to other central topics, such as selfhood, perception, attention and embodiment. At the same time, the articles display that consciousness is not just an isolated issue of philosophy of mind, but is bound to ontological, epistemological and moral discussions. Integrating historical inquiries into the systematic ones enables understanding the complexity and richness of conscious phenomena.

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