Page 1
Page 1
Zebrafish : Methods and protocols
Introduces new tools, models, and analytic insights that position the zebrafish even more strongly as an engine of discovery for developmental and disease biology. Beginning with a section exploring detailed methods for use of zebrafish to model a variety of human diseases, the book continues by illuminating the key ongoing role of the fish model in studies of the vertebrate nervous system, tools and approaches using zebrafish to study stem cell and regenerative biology, as well as techniques in genetics and genomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls.
Xylem : Methods and protocols
Provides detailed techniques used for the study and characterization of the plant vascular system, with a central focus on the xylem tissue. Chapters are organized in three main sections covering; analysis of xylem development, xylem characterization though imaging techniques, and analysis of the xylem composition. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Viral metagenomics : Methods and protocols
Expands on the previous edition with discussions about the latest viral metagenomics aspects covering a range of different specimens such as soil, freshwater, wastewater, fecal samples, blood plasma, clinical tissues, fungi, and herbarium samples. Chapters also look at different viral groups including archaeal viruses, eukaryotic viruses, phages, mycoviruses, and circular DNA viruses. Techniques required for studying the three viral metagenomic steps of samples processing, library construction, and analysis of data are also discussed. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Views on Microstructures in Granular Materials
This volume provides overview of the mechanics of granular materials, ranging from sparse media to soils. With chapters exploring state-of-the-art theoretical, experimental, and applied trends in the study of granular matter in various states, readers will be motivated to learn about the current challenges and potential avenues of exploration in this active area of research. Including a variety of perspectives, this volume will be a valuable reference for audiences in a number of fields.
Validation in Chemical Measurement
Validation of measurement methods has been used for a very ciated measurement uncertainty? The answer must be: no. long time in chemistry. It is mostly based on the examination There can never be a mechanism or recipe for producing - of a measurement procedure for its characteristics such as tomatically valid results because one can never eliminate precision, accuracy, selectivity, sensitivity, repeatability, re- theskills, the role and the responsibility of the analyst. producibility, detectionlimit, quantification limit and more. ISO 9000:2000, item 3. 8. 5 defines validation as confer- When focussing on quality comparability and reliability mation by examination and provision of objective evidence in chemical measurement.
Vaccine Design: Methods and Protocols ; Vol.2 : Vaccines for Veterinary Diseases
Guides readers through veterinary vaccines, vaccines for poultry, vaccines for farm animals, and vaccines for veterinary parasites. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Vaccine design : Methods and protocols ; Vol.1 : Vaccines for human diseases
Guides readers through an introductory section on future challenges for vaccinologists and the immunological mechanism of vaccines. Chapters focus on design of human vaccines for viral, bacterial, fungal, and parasitic diseases as well as tumor vaccines. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Turbulence, Dynamos, Accretion Disks, Pulsars and Collective Plasma Processes ; First Kodai-Trieste Workshop on Plasma Astrophysics Held at the Kodaikanal Observatory Kodaikanal, India, August 27 – September 7, 2007
It is well established and appreciated by now that more than 99% of the baryonic matter in the universe is in the plasma state. Most astrophysical systems could be approximated as conducting fluids in a gravitational field. It is the combined effect of these two that gives rise to the mind boggling variety of configurations in the form of filaments, loops , jets and arches. The plasma structures that cannot last for more than a second or less in a laboratory remain intact for astronomical time and spatial scales in an astrophysical setting. The case in point is the well known extragalactic jets whose collimation and stability has remained an enigma inspite of the efforts of many for many long years. The high energy radiation sources such as the active galactic nuclei again summon the coherent plasma radiation processes for their exceptionally large output from regions of relatively small physical sizes. The generation of magnetic field, anomalous transport of angular momentum with decisive bearing on star formation processes, the ubiquitous MHD turbulence under conditions irreproducible in terrestrial laboratories are some of the generic issues still awaiting a concerted effort for their understanding.
Trophoblasts : Methods and protocols
Explores the latest approaches used to assess trophoblast angiogenesis, transport function, cellular respirations, migration, and invasion. The chapters in this book cover topics such as various methods to study and manipulate primary trophoblast cells; strategies using liposomes to deliver biomolecules to trophoblasts; in vitro models of the placenta that emulate the cellular interactions in the 3D uterine environment; and models exploring heterogenous cell types and 3D-organoid structures that represent cell-to-cell interactions in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Transmembrane β-barrel proteins : Methods and protocols
Explores experimental strategies and protocols for the expression, assembly, characterization, and exploitation of transmembrane β-barrel proteins. Beginning with methodologies to study their assembly, the book continues with protocols for characterizing the landscape of transmembrane β-barrel protein interactions with other cellular factors, dissecting processes of protein transport in bacteria and mitochondria, examining structural characterization, determination, and prediction, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Therapeutic antibodies : Methods and protocols
Covers methods for studying, producing, and analyzing therapeutic antibodies, measuring their concentration, developing neutralizing antibodies for them, and for predicting and monitoring their therapeutic efficacy and clinical effects. These biologics are the fastest growing pharmaceutical drug group and have had tremendous clinical and scientific impact in cancer, autoimmune diseases, infectious diseases, and other immune-related diseases, making the content of this volume essential. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible methods, and tips on troubleshooting and avoiding known pitfalls.
The open handbook of linguistic data management
This handbook contains principles and methods for the management, archiving, sharing, and citing of linguistic research data-especially digital data-with an eye toward engaging growing trends in transforming linguistics into a more data-driven and reproducible scientific endeavor
The Local Langlands Conjecture for GL(2)
If F is a non-Archimedean local field, local class field theory can be viewed as giving a canonical bijection between the characters of the multiplicative group GL(1,F) of F and the characters of the Weil group of F. If n is a positive integer, the n-dimensional analogue of a character of the multiplicative group of F is an irreducible smooth representation of the general linear group GL(n,F). The local Langlands Conjecture for GL(n) postulates the existence of a canonical bijection between such objects and n-dimensional representations of the Weil group, generalizing class field theory. This conjecture has now been proved for all F and n, but the arguments are long and rely on many deep ideas and techniques. This book gives a complete and self-contained proof of the Langlands conjecture in the case n=2. It is aimed at graduate students and at researchers in related fields. It presupposes no special knowledge beyond the beginnings of the representation theory of finite groups and the structure theory of local fields. It uses only local methods, with no appeal to harmonic analysis on adele groups.
The Family, Medical Decision-Making, and Biotechnology : Critical Reflections on Asian Moral Perspectives
Opens with an exploration of the Confucian recognition of the family as an entity existing in its own right and which is not reducible to its members or their interests. As the essays in this volume show, this recognition of the family supports a notion of family autonomy that contrasts with Western individualistic accounts of proper medical decision-making.
The Achilles of Rationalist Psychology
How is it that the mind perceives the words of a verse as a verse and not just as a string of words? One answer to this question is that to do so the mind itself must already be unified as a simple thing without parts (and perhaps must therefore be immortal). Kant called this argument the Achilles, perhaps because of its apparent invincibility, and perhaps also because it has a fatal weak spot, or perhaps because it is the champion argument of rationalism. The argument and the problem it addresses have a long history, from the ancient world right up to the present.
Teratogenicity testing : Methods and protocols
Collects laboratory techniques and tests to assess the risks to embryo-fetal development from drug exposure during early developmental stages. Beginning with a section detailing basic principles of teratogenicity, the book continues with application of in vitro models for teratogenic screening to predict toxicity in embryonic animals and humans, as well as a variety of well-established in vivo animal model tests, which offer solutions for evaluating the efficacy and the teratogenicity of compounds in preclinical trials before human safety testing. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Tau protein : Methods and protocols
Explores the latest advancements and techniques to study Tau protein that include basic and advanced methods and protocols from in vitro assays to in vivo models that address the molecular and functional aspects of tau physiopathology and many of its related technical issues. The chapters in this book are organized into five parts: Part One describes conformational and functional studies of native tau protein using wet and non-wet lab protocols. Part Two looks at in vitro methods to monitor or control the formation of Tau oligomers and fibrils, and the fibrillization process. Part Three provides protocols for the characterization and in vitro introduction of post-translational modifications in Tau protein for further functional studies. Part Four describes analytical tools for the detection of Tau proteins under various forms, factors associated with Tau pathology, and MAPT gene studies. Finally, Part Five explores cellular and in vivo models for the investigations of Tau physiopathology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Systems biology
Part One looks at models in systems biology and parameters identification such as short peptide analysis, metastasis models, and metabolomics. Part Two covers computational methods in the study of organisms, and cancer non-linear dynamics. Part Three discusses critical transition states across Waddington’s like landscapes such as understanding cell differentiation through single-cell approaches and modeling mammary organogenesis from biological first principles. Part Four talks about specific fields of investigation including inborn errors of metabolism, system biology approach in epithelial-mesenchymal transition, and an approach to understanding how COVID-19 spreads in the population. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Synthetic biology : Methods and protocols
Provides new and updated techniques and applications associated with synthetic biology. Chapters guide readers through the creation and regulation of gene circuits, manipulation of biochemical pathways, genome editing and modification, creating genome language and computing, as well as molecular assembly.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Studies in Lie Theory : Dedicated to A. Joseph on his Sixtieth Birthday
Dedicated to Anthony Joseph, this volume contains surveys and invited articles by leading specialists in representation theory. The focus here is on semisimple Lie algebras and quantum groups.
