الصفحة 1
الصفحة 1
Neuronal cell death : Methods and protocols
Covers comprehensive methods on ways to assess structural and ultrastructural changes in the mitochondria, cytoskeleton, and microglia using state-of-the-art microscopy techniques including super-resolution imaging, electron microscopy, and ultra-high field MRI. The chapters in this book cover topics such as analysis of neurodegeneration in the post-mortem characterization of preclinical animal models, in vivo modeling in cell death in different model systems and brain organoids, single cell clonal analysis using Mosaic Analysis with Double Markers in genetic mouse models, and genome and proteomic methods for analysis of mRNA dynamics and quantitation of targeted peptides.
Neuroacanthocytosis Syndromes II
Awareness of neuroacanthocytosis disorders has increased significantly in recent years. There have been a number of important developments in the field since the publication of the first volume, Neuroacanthocytosis Syndromes. This book contains the latest research in this area. Recent advances have identified the range of mutations in the causative genes, shedding light on potential phenotypegenotype correlations. Studies of the proteins affected in these disorders have resulted in increased understanding of their functions and distribution. In vitro studies have identified potential protein interactions, which have important implications for pathophysiology. Work on erythrocyte membranes suggests mechanisms for the generation of acanthocytes. Animal models are being generated which will greatly facilitate understanding the role of gene mutations in humans, and provide the foundation for possible therapeutic interventions. In addition, advances in other neurodegenerative disorders, such as Huntington’s and Parkinson’s diseases, have implications for neuroacanthocytosis.
Mouse Models of Human Blood Cancers : Basic Research and Pre-clinical Applications
Although it remains an open question among some people whether mice and humans are similar in disease development, the laboratory mouse has emerged as the preeminent animal model for human diseases. This is underscored by the recently completed mouse and human genome projects, which have revealed that mice and humans share the vast majority of their genes, and thus get many of the same diseases, and for the same reasons. Emphasizing why mouse models are valuable in vivo systems for understanding disease mechanisms and developing therapeutic strategies for human blood cancers, "Mouse Models of Human Blood Cancers: Basic Research and Pre-clinical Applications," edited by Shaoguang Li, aims on presenting thorough analyses of the pathological features and the molecular bases of several major types of blood cancer and to describe translational research using mouse cancer models.
Memory B-cells : Methods and protocols
Details in methods to assess memory B cell formation and function in mice and humans. Chapters guide readers through tetramer-based methods to assess antigen-specific memory B cell dynamics in humans and mice in different vaccine, next-generation deep-sequencing, single-cell techniques to assess epigenomic, VDJ landscapes, lymph node aspirates from humans, advanced imaging, murine models to determine memory B cells formation, and bioinformatic techniques and in silico modelling of memory b cell formation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Innovative Medicine : Basic Research and Development
Devoted to innovative medicine, comprising the proceedings of the Uehara Memorial Foundation Symposium 2014. It remains extremely rare for the findings of basic research to be developed into clinical applications, and it takes a long time for the process to be achieved. The task of advancing the development of basic research into clinical reality lies with translational science, yet the field seems to struggle to find a way to move forward. To create innovative medical technology, many steps need to be taken: development and analysis of optimal animal models of human diseases, elucidation of genomic and epidemiological data, and establishment of “proof of concept”.
In Vivo Models of Inflammation ; Vol. II
In Vivo Models of Inflammation (Vol. 2) provides biomedical researchers in both the pharmaceutical industry and academia with a description of the state-of-the-art animal model systems used to emulate diseases with components of inflammation.
In Vivo Models of Inflammation ; Vol. I
In Vivo Models of Inflammation (Vol. 1) provides biomedical researchers in both the pharmaceutical industry and academia with a description of the state-of-the-art animal model systems used to emulate diseases with components of inflammation.
In vivo Models of HIV Disease and Control
An AIDS vaccine is still elusive and HIV treatment continues to develop multidrug resistance at alarming rates. Because of the similarities between HIV and immune deficiency infections in a variety of animals, it is only natural that scientists use these animals as models to study pathogenesis, treatment, vaccine development and many other aspects of HIV. Part of the series Infectious Agents and Pathogenesis, this volume reviews the immune deficiency virus in a variety of hosts. Pathogenesis, vaccine and drug development, epidemiology, and the natural history of the monkey, mouse, cat, cow, horse, and other animal viruses are detailed and compared to HIV. Also included are chapters on the history and future of animal models, as well as a chapter on ethical and safety considerations in using animal models for AIDS studies.
Human Cytomegalovirus
The golden age of cytomegalovirus research was ushered in during the late 1970s and early 1980s by a set of powerful new technologies that included restriction enzymes, DNA cloning, DNA sequencing, and open reading frame prediction. The genetic manipulation and propagation of novel CMV strains was accelerated with the app- cation of bacterial artificial chromosome technology. Today, we still struggle to understand the full spectrum of disease associated with human CMV. To the molecular biologist, CMV is a master of regulation in the eukaryotic cell where it either replicates or remains latent. To the immunologist, CMV is a master of immune evasion with tools to escape both the innate and acquired immune responses. The use of animal models with non-human CMVs has become significantly more sophisticated and tied to a more certain understanding of the interrelationships of non-human and human CMV genes.
Hormonal carcinogenesis V
Information gathered from cell-free systems, cell cultures, animal models, and human studies, together will (1) provide important insights to our understanding of hormonal cancer causation, development, and prevention; (2) be the primary objective of these Symposia.
High Density Lipoproteins : From Biological Understanding to Clinical Exploitation
In this Handbook of Experimental Pharmacology on “High Density Lipoproteins – from biological understanding to clinical exploitation” contributing authors (members of COST Action BM0904/HDLnet) summarize in more than 20 chapters our current knowledge on the structure, function, metabolism and regulation of HDL in health and several diseases as well as the status of past and ongoing attempts of therapeutic exploitation.
Handbook of In Vivo Chemistry in Mice : From Lab to Living System
The Handbook of In Vivo Chemistry in Mice: From Lab to Living System introduces readers to general information about live animal experiments and detection methods commonly used for these animal models. It focuses on chemistry-based techniques to develop selective in vivo targeting methodologies, as well as strategies for in vivo chemistry and drug release.
Handbook of cardiac anatomy, physiology, and devices
Because of rapid growth in the medical device industry-which today enjoys revenues as large as those of the automobile industry-as well as in our understanding of cardiac disease processes, biomedical engineers must cope with a rising tide of highly complex information. In Handbook of Cardiac Anatomy, Physiology, and Devices, leading experts from the University of Minnesota's renowned Lillehei Heart Institute and scientists from Medtronic, Inc. have compiled a concise-yet detailed and comprehensive-reference source on the anatomic features, underlying physiologic mechanisms, and treatments for diseases of the heart. Here, active bioengineers working on cardiac devices will find the complete critical background they need to understand cardiac pacing, defibrillation, cardiac repair using stem cell therapy, robotics, less invasive cardiac surgery, biventricular pacing, cardiac bioenergetics, and more. Insightful chapters address animal models for cardiac research, cardiac mapping systems, heart valve disease, ventricular assist devices, and genomics-based tools and technology.
Germ cell development : Methods and protocols
Explores techniques for the isolation, purification, and establishment of in vitro germ cell (GS) systems at different stages of development in a variety of organisms, including chickens, mice, rats, and humans. The book additionally describes cutting-edge analytic and informatic tools to study germ cell development at the single cell level and meiotic recombination. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Fundamentals of Space Medicine
Topics discussed in this book include: adaptation of sensory-motor, cardio-vascular, bone, and muscle systems to the microgravity of spaceflight; psychological and sociological issues of living in a confined, isolated, and stressful environment; operational space medicine, such as crew selection, training and in-flight health monitoring, countermeasures and support; results of space biology experiments on individual cells, plants, and animal models; and the impact of long-duration missions such as the human mission to Mars. The author also provides a detailed description of how to fly a space experiment, based on his own experience with research projects conducted onboard Salyut-7, Mir, Spacelab, and the Space Shuttle. Now is the time to look at the future of human spaceflight and what comes next. The future human exploration of Mars captures the imagination of both the public and the scientific community. Many physiological, psychological, operational, and scientific issues need to be solved before the first crew can explore the enigmatic Red Planet. This book also identifies the showstoppers that can be foreseen and what we need to learn to fully understand the implications and risks of such a mission.
Food allergens : methods and protocols
Provides a comprehensive collection of methods and protocols in food allergy and food allergens studies. The selected protocols explore the study of food allergens, from recombinant production, purification procedures, IgE and T cell epitopes characterization, to allergen structure description, cellular responses, and tolerance induction, through a variety of techniques and animal models.
Flow cytometry : Principles and applications
Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers. The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. Throughout Flow Cytometry: Principles and Applications, well-informed expert contributors present theoretical descriptions and practical protocols on this important and complex laboratory technique and its applications. Immunologists and Hematologists in the field of pathology, as well as biological researchers working with both human and animal models will appreciate the simple, clear-cut style in which principles and protocols in this volume are presented, and will refer to this book time and time again for clear and easy-to-follow protocols.
Ferroptosis : Methods and protocols
A comprehensive collection of experimental protocols for investigating ferroptosis in different systems, including cultured cells, animal models, and human tissues. The techniques covered in this book look at various aspects of ferroptosis ranging from the detection of lipid peroxidation to the measurement of glutathione peroxidase activity and the evaluation of mitochondrial morphology. Chapters also discuss basic molecular biology methods such as quantitative PCR and immunoblotting, and advanced imaging techniques such as transmission electron microscopy and confocal fluorescence microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Endocrinology : Basic and Clinical Principles
For this new edition of Conn & Melmed's Endocrinology: Basic and Clinical Principles, a panel of distinguished scientists and clinicians has completely rewritten every chapter to reflect the latest advances in our understanding of the endocrine system. Maintaining the original goal of the first edition to integrate the basic science of endocrinology with its physiological and clinical principles, the authors have succinctly summarized in 450 pages the latest findings on hormone secretion and hormone action, as well as all of the most recent insights into the physiology and pathophysiology of hormonal disorders. Coverage extends across the entire spectrum of endocrinology-from mammalian cells, plants, and insects to animal models and human diseases-with much increased coverage of diabetes and metabolism. Highlights include cutting-edge discussions of appetite disorders, obesity, reproductive failure, control of thyroid function, hormone action in humans and the lower species, and the mechanisms subserving hormone secretion.
Effector-triggered immunity : methods and protocols
Presents a collection of protocols to study effector-triggered immunity (ETI) in both plants and animals from eminent groups in the field. Cover topics such as genetic manipulation of plant and animal pathogens, host cells, and the analysis of key host responses; and techniques used for the analysis of inflammasome activation, cell death pathways, and mitochondria damage in response to pathogens. All of these topics cover a broad spectrum of immunological, biochemical, cell biological, and structural biology approaches to examine ETI.
