Page 1
Page 1
Receptor Binding Techniques
Expands upon the methods and techniques used for studying receptors in silico, in vitro and in vivo. Comprehensive chapters describe how to use online resources for experimental research such as prediction of receptor-ligand interactions and mine the IUPHAR receptor database. Classical techniques of radioligand binding, quantitative autoradiography and their analyses are complemented by the use of immunocytochemistry for the cellular localization of receptor protein and hybridization to detect receptor mRNA. Protocols using fluorescent labeled ligands are described to visualise receptors in living cells, their interaction with beta-arrestin to measure ligand-induced internalisation and green fluorescent protein to study trafficking. Non-radioactive, chemiluminescent cAMP and arrestin assays facilitate the identification of novel ‘biased agonists’. Detailed methods are provided for in vivo imaging of receptors using positron emission tomography (PET).
PET : Physics, Instrumentation, and Scanners
Derived from the critically acclaimed reference PET: Molecular Imaging and Its Biological Applications, edited by Michael E. Phelps, Ph.D., this handbook provides a clear and concise introduction to the physics and instrumentation aspects of PET imaging. Comprised of select material from the parent volume, this book is gives the reader a solid understanding of PET fundamentals, including how PET data are collected and formed into an image. Other topics include basic physics, detector technology used in modern PET scanners, data acquisition, 3-D reconstruction, and methods for evaluating the performance of PET systems.
