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Molecular Diagnostic PCR Handbook
PREFACE The Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture is involved in agricultural research and development and assists Member States of FAO and IAEA in improving strategies to ensure food security through the use of nuclear techniques and related biotechnologies, where such techniques have a valuable and often unique role. In particular, molecular diagnostic methods have rapidly evolved in the past twenty years, since the advent of the Polymerase Chain Reaction (PCR). They are used in a wide range of agricultural areas such as, improving soil and water management; producing better crop varieties; diagnosing plant and animal diseases; controlling insect pests and improving food quality and safety. The uses of nucleic acid-directed methods have increased significantly in the past five years and have made important contributions to disease control country programmes for improving national and international trade. These developments include the more routine use of PCR as a diagnostic tool in veterinary diagnostic laboratories. However, there are many problems associated with the transfer and particularly, the application of this technology. These include lack of consideration of: the establishment of quality-assured procedures, the required set-up of the laboratory and the proper training of staff. This can lead to a situation where results are not assured. This book gives a comprehensive account of the practical aspects of PCR and strong consideration is given to ensure its optimal use in a laboratory environment. This includes the setting-up of a PCR laboratory; Good Laboratory Practice and standardised of PCR protocols.
Mid-Infrared Coherent Sources and Applications
Coherent sources of mid-infrared (mid-IR) radiation are of great interest for a wide range of scienti?c and technological applications from spectroscopy and frequency metrology to information technology, industrial process control, pho- chemistry, photobiology and photomedicine. The mid-IR spectrum, which may be de?ned as wavelengths beyond ?2µm, covers important atmospheric windows, and numerous molecular gases, toxic agents, air, water, and soil pollutants, c- ponents of human breath, and several explosive agents have strong absorption ?ngerprints in this region. The development of practical coherent solid-state sources in the mid-IR can thus provide indispensable tools for a variety of - plications in environmental monitoring and pollution control, detection of water and soil contaminants, food quality control, agriculture and life sciences, and n- invasive disease diagnosis and therapy through breath analysis.
Immobilisation of DNA on Chips II
DNA chips are gaining increasing importance in different fields ranging from medicine to analytical chemistry with applications in the latter in food safety and food quality issues as well as in environmental protection. In the medical field, DNA chips are frequently used in arrays for gene expression studies to identify diseased cells due to over- or under-expression of certain genes, to follow the response of drug treatments, or to grade cancers), for genotyping of individuals, for the detection of single nucleotide polymorphisms, point mutations, and short tandem reports, or moreover for genome and transcriptome analyses in the quasi post-genomic sequencing era. Furthermore, due to some unique properties of DNA molecules, self-assembled layers of DNA are promising candidates in the field of molecular electronics.the main focus of these two volumes is on the immobilization chemistry, considering the various aspects of the immobilization process itself, since different types of nucleic acids, support materials, surface activation chemistries and patterning tools are of key concern.
Fruit Manufacturing : Scientific Basis, Engineering Properties, and Deteriorative Reactions of Technological Importance
The fruit processing industry is a major global business. While basic principles of fruit processing have shown only minor changes over the last years, major improvements continually occur. More efficient equipment is capable of converting huge quantities of fruits into pulp, juice, dehydrated, frozen and refrigerated products, etc. that make possible the preservation of products for year-round consumption. Fruit processing and storage involve physical and chemical changes that negatively modify fruit quality. The industry’s ability to provide a nutritious and healthful fruit product to the consumer is highly dependent on the knowledge of the quality modifications that occur during the processing.
Food Safety and Ways to Prevent Life-Threatening Pollutants
The fears regarding food safety started long time ago, and till this very moment of ours, the international organizations and INGOs pay lots of hard work to minimize and achieve reduction in the effects and the suffering caused to people around the world by contaminated foods each year. This research talks about the main patterns of food contaminants, and they are chemical, physical, biological, and last but not least the contamination caused by radioactive substances. It is also important to talk about the methods of precaution and protection against the diseases and illnesses produced by contaminated foods, as well about the role of INGOs which are dedicated and specialized in law and regulations enactment for bounding the incidence of food contamination in the world, for raising the public awareness towards food safety, and to keep up with the latest updates of events. This research also contains vast Information about Covid-19 and its spreading process via contaminated food.
Chemical and functional properties of food components
Described the contents of food raw materials and products, the chemistry/biochemistry of food components, as well as the changes occurring during post-harvest storage and processing affecting the quality of foods. Discusses the role of chemical compounds in the structure of raw materials and the formation of different attributes of food quality, including nutritional value, safety, and sensory properties. It contains four new chapters: “Non-Protein Nitrogenous Compounds”; “Prooxidants and Antioxidants in Food”; “Non-Nutritive Bioactive Compounds in Food of Plant Origin”; and “Analytical Methods Used for Assessing the Quality of Food Products.”
Case Studies in Food Safety and Environmental Health
The ISEKI-Food book series is a collection where various aspects of food safety and environmental issues are introduced and reviewed by scientists specializing in the field. In all of the books a special emphasis is placed on including case studies applicable to each specific topic. The books are intended for graduate students and senior level undergraduate students as well as professionals and researchers interested in food safety and environmental issues applicable to food safety.
A Portrait of State-of-the-Art Research at the Technical University of Lisbon
This book contains the edited version of the invited lectures that were delivered by prominent researchers at UTL. This book brings together in a review manner a comprehensive summary of high quality research contri- tions across basic and applied sciences. The contributing papers are organized around the following major areas: – Emergent areas (Nanosciences, Quantic Computations and Infor- tion, Risk and Volatility in Financial Markets); – Basic Sciences (Mathematics, Physics, Chemistry and Materials); – Social Sciences, Economics and Management Sciences; – Life Sciences and Biotechnology; – Engineering and Technologies – Nature, Environment and Sustainability; – Public Health, Food Quality and Safety; – Health and Sport Sciences; – Urbanism, Transports, Architecture, Arts and Design.
