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Adult stem cells : Methods and protocols
Expands on the previous edition with updated chapters covering a broader range of tissues and techniques pertaining to stem cell technologies. The chapters also cover topics such as the generation of iPSC-derived cells unique to the individual human genome addressing the possibility of more personalized clinical applications to an individual with a specific degenerative disease; and the use of nanoparticles such as 3D scaffolds and biomaterials as a means of improving stem cell viability after transplantation in the host tissue. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Adipose-derived stem cells : Methods and protocols
Discusses of the latest technology and advancements in the field of human-derived ASC and SVF. The chapters in this book are organized into four parts. Part One focuses on human ASC’s isolation, characterization, and differentiation. Part Two describes the isolation and characterization of ASC and SVF from canine, feline, and murine tissues. Part Three looks at hydrogels, scaffolds, and microphysiological systems, and Part Four talks about the new assays and applications using ASC. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
