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It summarizes the year’s progress in fluorescence, with authoritative analytical reviews specialized enough to be attractive to professional researchers, yet also appealing to the wider audience of scientists in related disciplines of fluorescence. Reviews in Fluorescence 2005 topics include: - RNA folding and RNA-protein binding analyzed using fluorescence. - Progress in Lanthanides as luminescent probes. - The combined use of fluorescence spectroscopy and X-Ray crystallography to elucidating structure and dynamics of proteins. - Tapered fibers for cell studies. - Fluorescence-based ophthalmic glucose monitoring for diabetics. - High-throughput tissue image cytometry. - DNA condensation using FCS. - Multidimensional TCSPC.