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In all organs of the body proteases have critical roles to play both in normal development and functioning and in disease states. The brain is no exception to this, with proteases having emerging roles in synaptic plasticity, memory, neurodegenerative disorders such as Alzheimer’s, Parkinson’s and prion diseases, ischemia and traumatic brain injury, inflammatory and infectious diseases, and tumour progression. Proteases in the Brain brings together a wide range of topics under this central theme and highlights the large number of proteases involved in these normal and disease processes.