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This book constitutes the refereed proceedings of the 16th International Symposium on Algorithms and Computation, ISAAC 2005, held in China in 2005. The 112 revised full papers presented were carefully reviewed and selected from 549 submissions. The papers are organized in topical sections on computational geometry, computational optimization, graph drawing and graph algorithms, computational complexity, approximation algorithms, internet algorithms, quantum computing and cryptography, data structure, computational biology, experimental algorithm mehodologies and online algorithms, randomized algorithms, parallel and distributed algorithms, graph drawing and graph algorithms, computational complexity, combinatorial optimization, computational biology, computational complexity, computational optimization, computational geometry, approximation algorithms, graph drawing and graph algorithms, computational geometry, approximation algorithms, graph drawing and graph algorithms, and data structure.