Receptor binding techniques / Anthony P. Davenport
Publication year: 2005
ISBN: 978-1-59259-927-1
Internet Resource: Please Login to download book
Coveres mining from curated databases, identifying novel receptors by high throughput screening, molecular methods to identify mRNA encoding receptors, radioligand binding assays and their analysis, quantitative autoradiography, and imaging receptors by positron emission tomography (PET). Highlights include phenotypic characterization of receptors in knockout mice, imaging receptors using green fluorescent protein and fluorescent resonance energy transfer, and quantitative analysis of receptor mRNA by TaqMan PCR. These book equips the researcher with techniques for exploring the unprecedented number of new receptor systems now emerging and the so-called "orphan" receptors whose activating ligand has not been identified.
Subject: Biochemistry, Binding Sites, Binding sites (Biochemistry), Cell receptors Research, Cell receptors Research Laboratory manuals, Complexes récepteur-ligand Recherche Manuels de laboratoire, Dosage radiocompétitif Manuels de laboratoire, Laboratory manuals, Moleculaire biologie (algemeen), Molecular Biology (General), Radioligand Assay methods, Radioligand assay, Radioligand assay Laboratory manuals, Receptor-ligand complexes Research Laboratory manuals, Receptors, Drug isolation & purification, Récepteurs cellulaires Recherche Manuels de laboratoire, Cell Biology, Sites actifs (Biochimie), Biochemical receptors
