Publication year: 2015
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This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds.
Subject: Biomedical and Life Sciences, Plant Breeding, Biotechnology, Biological Techniques, Nucleic Acid Chemistry, Crop improvement, DNA extraction, Induced mutations, Low-cost assays, Mutant germplasm characterization, Nucleotide variation in plant genomes, PCR amplification