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The adsorption of proteins at interfaces plays a role in many fields, suchas health, food, environment and analysis. Fundamental aspects are usefulwhen considering applications. We focus here especially on solid–liquidinterfaces and present a few fundamental studies regarding adsorption ki-netics and conformational changes, and examples of applications to sensorsand membranes.The first part is dedicated to fundamental studies performed using op-tical waveguide lightmode spectroscopy, as an example of a technique thathas the advantage of not requiring labelled proteins, but is limited to spe-cific supports.The second part includes different bench techniques that were devel-oped to improve the sensitivity of the characterisation of the orientationand structure of the proteins at interfaces.