Receptor Binding Techniques / Brinda K. Rana, Philip E. Bourne, Paul A. Insel, Anthony P. Davenport
Publication year: 2012
ISBN: 978-1-61779-909-9
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Expands upon the methods and techniques used for studying receptors in silico, in vitro and in vivo. Comprehensive chapters describe how to use online resources for experimental research such as prediction of receptor-ligand interactions and mine the IUPHAR receptor database. Classical techniques of radioligand binding, quantitative autoradiography and their analyses are complemented by the use of immunocytochemistry for the cellular localization of receptor protein and hybridization to detect receptor mRNA. Protocols using fluorescent labeled ligands are described to visualise receptors in living cells, their interaction with beta-arrestin to measure ligand-induced internalisation and green fluorescent protein to study trafficking. Non-radioactive, chemiluminescent cAMP and arrestin assays facilitate the identification of novel ‘biased agonists’. Detailed methods are provided for in vivo imaging of receptors using positron emission tomography (PET).
Subject: Biochemistry, Cell receptors, G-protein-coupled receptors, Immunocytochemistry, PET scanners, Radioligand binding, Anatomical distribution, Image analysis, ligand-receptor binding, mRNA, Orphan receptors, Phosphor screens, Quantitative autoradiography
