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Laurus nobilis is an evergreen plant belonging to the Lauraceae family, native to Southern Europe and the Mediterranean area. This is the report on the composition and bioactivity of laurel essential oil (EO) from leaves and extraction essential oil by two solvents (water and ethanol 69%) using by two devices (Clevenger and evaporator) then we apply the effect of oil and extract on the bacteria. there are some differences between the extraction by water and ethanol . The antibacterial properties of laurel EOs were examined according to the agar well diffusion method The leaf EO showed antibacterial activities against almost all strains of the microorganisms tested, whereas the twig EO was only able to inhibit Staphylococcus aurous and Escherichia coli were the bacterial strains that showed the highest resistance to the laurel EO. The results can benefit the EO industry and bio pesticide development.