Misrabie R; Hamad E. and Ismail M.T.

Researchers

Journal of Laboratory Diagnosis, Volume 5, Issue 5, Shawwal 1430 - October 2009

Published in

Background: Rotavirus is one of the most important viruses that causes diarrhea. This name came from its shape under electronic microscope which looks like a wheel. This virus was discovered in humans in 1973 by Bishop and his assistants in Australia by taking biopsy from gastro intestinal tract of babies injured by stomach or intestine high inflammation. It was noticed that it resists heat, lyses yeasts, and disinfectants. It keeps on its replication on pH = 3-10. This virus infects the gastro intestinal tract and replicates in it at high level, so it injures transportation mechanisms which makes the patient lose salt, glucose, and water causing diarrhea, vomiting, etc…. This inflammation presents in babies at a high level which cause dryness and a big problem in ions balance, otherwise this symptoms usually present at low level. PCR is one of the technologies that diagnose and identify Rota virus because it is used widely in molecular biology.

Aim: This research focused on study and identified Rota virus in ministry of health in Syria because of the lack of researches there, and compare results with others in developing countries.
Methods: We identify Rota virus in Syria by PCR for diarrhea children from 2 months till 2 years old (200 babies). EIA was used also and we reached the relationship between heat, diarrhea, vomiting, dryness, oral fecal treatment and infection percentage.
Results and Conclusion: The study showed 129 infected states from 200, and infection percentage was 64.5%. The high one was 10.85% in 2 years old babies. In June 2008 reached 74.07% but it reached 60% in June 2007 and that means that this percentage rises year by year. So we have to make big researches about methods used to protect our children from this virus.

Abstract

http://scla.org.sy/magazine/issues/5_5/297.html

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