Researchers

1. Ramadan; M. T. Esmail and S. Soukkaria

Published in

Damascus University Journal of Basic Sciences, Volume 29, Issue 2, 2013

Abstract

Fungal infections caused by Aspergillus species generally are occupying a second place among invasive fungal infections in the world, especially A.fumigatus, which is considered the main cause of invasive Aspergillosis (IA). Although IA rarely infects immunocompetent individuals, however, it can lead to death in immunocompromised patients. Therefore, it is necessary to diagnose the infection early in order to treat the disease efficiently. However, the conventional diagnostic tools, currently used to detect infections, have low sensitivity and reliability. Polymerase chain reaction (PCR) technology distribution as a molecular and high sensitive technology has allowed us to make comparative study between sensitivity of traditional currently used diagnostic method and Nested-PCR, the result of the study of sputum samples that experimentally infected with different concentrations of A.fumigatus spores ramping from 10 to10⁶ spore/ml, have high sensitivity and specificity of Nested-PCR in detecting the lower concentrations, comparing with traditional diagnostic method (culture on Sabouraud media) that were negative in all concentrations.

Keywords: Invasive Aspergillosis, A. fumigates, Nested PCR, Genomic DNA.

Link to read full paper

http://www.damascusuniversity.edu.sy/mag/asasy/images/stories/2-2013/411-426.pdf