الصفحة 1
الصفحة 1
Neutrophil : Methods and Protocols
Neutrophils, the most abundant white cells in humans, serve as the primary cellular defense against infection. This volume provides a concise set of protocols for assessing basic neutrophil functions and investigating specialized areas in neutrophil biology. Each of the protocols is written by leading researchers in the field and includes hints for success, as well as guidance for troubleshooting. Part I contains overviews of neutrophil biology, function, and disorders. Part II describes commonly used methods to isolate neutrophils from humans and other animal species. Part III details methods for investigating chemotaxis, transmigration, phagocytosis, and bactericidal activity. Part IV includes protocols that measure neutrophil apoptosis, calcium signal transduction, degranulation and detection of cytoplasmic granules, gene expression, transcription factors, and apoptosis. Part V provides multiple assays for measuring production of intracellular and/or extracellular reactive oxygen species, and a method that details use of the cell-free NADPH oxidase assay, an iconic assay for studies of the neutrophil NADPH oxidase. Part VI provides chapters that describe how to analyze formation and function of neutrophil extracellular traps.
Neuroprotection : Method and protocols
Contains cutting-edge molecular biology methods on neuroprotective mechanisms and specific preclinical models of the CNS injury, iseases and planning translation. Chapters guide readers through neuropathology, neuroprotection, Alzheimer’s disease, amyotrophic lateral sclerosis, ALS, Huntington’s disease , multiple sclerosis, Parkinson’s disease, spinal cord injury, traumatic brain injury, and ischemic brain injury. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Neurobiology : Methods and protocols
Serves as a guide for students in the field of neurobiology, and be a bridge between basic science researchers, doctors, and surgeons in clinical practice. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Network+ Guide to Networks
It thoroughly prepares you for success on CompTIA's Network+ N10-007 certification exam with fully mapped coverage of all objectives, including protocols, topologies, hardware, network design, security and troubleshooting. Virtualization-based projects give you experience working with a wide variety of hardware, software, operating systems and device interactions, while "On the Job" stories, Applying Concepts activities, and Hands-On and Capstone Projects let you explore concepts in more depth. MindTap Networking offers additional practice and certification prep.
Mucins : Methods and protocols
Explores the latest advancements in mucin research. The chapters in this book are organized into 8 parts and cover a wide range of topics such as mucin extraction, isolation, physicochemical property analysis, and experimental methods. The chapters also discuss the origins of mucins in jellyfish, feces, saliva and salivary glands, bronchi, stomach, and cervical tract; organic synthesis of peptides glycosylated at specific sites; analysis of mucin gene expression and methylation-specific electrophoresis of genes; imaging of mucin networks by AFM; and experimental methods using supported molecular matrix electrophoresis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Microsoft Content Management Server Field Guide
Microsoft Content Management Server Field Guide provides simple crisis-management instructions for intermediate- to advanced-level network administrators who work in the trenches. It offers practical procedural information, reference materials, and focused presentations. This book also features self-contained topics so you do not have to look further to obtain prerequisite information.
Micropropagation methods in temporary immersion systems
Presents vital techniques for the commercial micropropagation of plants. It explores numerous micropropagation protocols in different types of temporary immersion systems for plant species with agricultural, medicinal, ornamental, and forestry interest, detailing the type of bioreactor and the optimal parameters necessary to guarantee obtaining the largest number of commercial propagules. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Microfluidics diagnostics : Methods and protocols
Explores a wide range of microfluidic-based approaches that exploit the unique features of microfluidic devices, which hold significant potential in the field of diagnosis. Beginning with a section on microchips for sample preparation, including cell-free DNA, exosomes, and cells, the book continues by covering protein marker analysis and detection, single-cell analysis, analysis of bacteria and viruses, as well as human cell-based culture and analysis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Memory B-cells : Methods and protocols
Details in methods to assess memory B cell formation and function in mice and humans. Chapters guide readers through tetramer-based methods to assess antigen-specific memory B cell dynamics in humans and mice in different vaccine, next-generation deep-sequencing, single-cell techniques to assess epigenomic, VDJ landscapes, lymph node aspirates from humans, advanced imaging, murine models to determine memory B cells formation, and bioinformatic techniques and in silico modelling of memory b cell formation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Iron metabolism : Methods and protocols
Explores classical and cutting-edge methods optimized and validated to analyze various aspects of iron metabolism, from in vitro to multi-organ level. Opening with a section on basic iron metabolism methods, the book continues with methods applicable to a variety of systems, ranging from bacteria to cultured mammalian cells and tissues, with a focus on cellular heme and iron-sulfur cluster species, as well as mitochondrial iron and its derivatives. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Immunogenetics: Methods and Protocols
Explores techniques for working in the field of immunogenetics, i.e. fundamental and translational research into the adaptive immune receptor repertoire. Many chapters are dedicated to lab protocols, bioinformatics, and immunoinformatics analysis of high-resolution immunome analysis, exemplified by numerous applications. Additionally, the newest technological variations on these protocols are discussed, including non-amplicon, single-cell, and cell-free strategies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Immune receptors : Methods and protocols
Explores immune cell receptors that are used in the detection of microbes, either by binding directly to non-self molecules or through indirectly sensing microbe-associated cellular disturbances. The covers methods for studying receptor-ligand interactions at both molecular and cellular levels; methods to create and characterize novel antibody reagents; and methods to characterize the molecular processes that lead to adaptive receptor maturation. This book also contains chapters that look at high-throughput strategies that describe the diversity of immune receptors and cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Immune mediators in cancer : Methods and protocols
Provides a comprehensive collection of classic and cutting-edge methodologies as well as bioinformatics and genome-editing approaches that are used to quantify immune mediators and analyze their function and biological activity in cancer cells and tissues. Beginning with a section on the detection of immune mediators in samples, the volume continues with sections covering cytokine bioassays, the expression and regulation of immune mediators in cancer cells, and methods to navigate the enormous datasets created by modern DNA and RNA sequencing and proteomic technology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Host-pathogen interactions : Methods and protocols
Serves as multidisciplinary compendium of approaches and techniques employed to analyze the role of different molecules, processes, or strategies used by different guests to survive and proliferate in their associations with eukaryotic hosts. Beginning with animal-pathogen interactions, the book then continues with chapters exploring virus-host interactions, plant-microbe interactions, as well as different molecular techniques that were initially applied to non-pathogenic interactions but can be adapted to study other host-pathogen associations. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips for troubleshooting and avoiding known pitfalls.
Histones : Methods and protocols
Provides new and updated chapters on histones for molecular biologists, biochemists and geneticists. Chapters cover protein complexes that modify chromatin either by adding or removing post-translational modifications, or by exchanging histone variants within the nucleosome. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls.
High Performance SQL Server " Consistent Response for Mission-Critical Applications
An excellent complement to query performance tuning books and provides the other half of what you need to know by focusing on configuring the instances on which mission-critical queries are executed. You will: Understand SQL Server's database engine and how it processes queries / Configure instances in support of high-throughput applications / Provide consistent response times to varying user numbers and query volumes / Design databases for high-throughput applications with focus on performance / Record performance baselines and monitor SQL Server instances against them / Troubleshot and fix performance problems
Hands-On Software Architecture with Java - Learn key architectural techniques and strategies to design efficient and elegant Java applications
Starts with the fundamentals of architecture and takes you through the basic components of application architecture. You'll cover the different types of software architectural patterns and application integration patterns and learn about their most widespread implementation in Java. You'll then explore cloud-native architectures and best practices for enhancing existing applications to better suit a cloud-enabled world. Later, the book highlights some cross-cutting concerns and the importance of monitoring and tracing for planning the evolution of the software, foreseeing predictable maintenance, and troubleshooting. The book concludes with an analysis of the current status of software architectures in Java programming and offers insights into transforming your architecture to reduce technical debt.
Germ cell development : Methods and protocols
Explores techniques for the isolation, purification, and establishment of in vitro germ cell (GS) systems at different stages of development in a variety of organisms, including chickens, mice, rats, and humans. The book additionally describes cutting-edge analytic and informatic tools to study germ cell development at the single cell level and meiotic recombination. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Foodborne bacterial pathogens : Methods and protocols
This updated volume presents a compilation of various representative techniques and approaches currently used to study bacterial foodborne pathogens. Chapters guide the reader through bacterial pathogen detection and quantification in food, molecular, phenotypic, metabolic characterization of food pathogens, and ecology of foodborne bacterial pathogens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Ferroptosis : Methods and protocols
A comprehensive collection of experimental protocols for investigating ferroptosis in different systems, including cultured cells, animal models, and human tissues. The techniques covered in this book look at various aspects of ferroptosis ranging from the detection of lipid peroxidation to the measurement of glutathione peroxidase activity and the evaluation of mitochondrial morphology. Chapters also discuss basic molecular biology methods such as quantitative PCR and immunoblotting, and advanced imaging techniques such as transmission electron microscopy and confocal fluorescence microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
