الصفحة 1
الصفحة 1
Nuucleic acids in medicinal chemistry and chemical biology : Drug development and clinical applications
Delivers a comprehensive overview of the chemistry and biology of nucleic acids and their therapeutic applications. The book emphasizes the latest research in the field, including new technologies like CRISPR that create novel possibilities to edit mutated genes at the genomic DNA level and to treat inherited diseases and cancers. The authors explore the application of modified nucleosides and nucleotides in medicinal chemistry, a variety of current topics on nucleic acid chemistry and biology, nucleic acid drugs used to treat disease, and more. They also probe new domains of pharmaceutical research, offering the reader a wealth of new drug discovery opportunities emerging in this dynamic field
Nucleic Acids Hybridization : Modern Applications
Several approaches, such as microarray hybridization, have become extremely popular tools for specialists in biochemistry and biomedicine, while the potential of many other advantageous techniques seems to be underestimated. Written by an international team of authors, this book details the current state-of-art in hybridization techniques. Coverage includes experimental protocols along with comprehensive and detailed method descriptions.
Nucleic Acids and Proteins in Soil
With millions of different bacterial species living in soil, the microbial community is extremely complex, varying at very small scales. Microbe-driven functions are essential for most processes in soil. Thus, a better understanding of this microbial diversity will be invaluable for the management of the various soil functions. Nucleic Acids and Proteins in Soil combines traditional approaches in soil microbiology and biochemistry with the latest techniques in molecular microbial ecology. Included are methods to analyse the presence and importance of nucleic acids and proteins both inside and outside microbial cells, the horizontal gene transfer which drives bacterial diversity, as well as soil proteomes. Further chapters describe techniques such as PCR, fingerprinting, the challenging use of gene arrays for structural and functional analysis, stable isotope probing to identify in situ metabolic functions, and the use of marker and reporter genes in soil microbial ecology.
NMR in Biological Systems : From Molecules to Human
During teaching NMR to students and researchers, we felt the need for a text-book which can cover modern trends in the application of NMR to biological systems. This book caters to the needs of: (i) graduate students who mostly learn such techniques from senior post-docs in the laboratory; (ii) those who are not experts in NMR but wish to understand if a particular problem in animal, plant, medical and pharmaceutical sciences can be answered by NMR; and (iii) those who are experts in chemistry and biochemistry and wish to know how NMR can provide them information on structural or functional aspect of proteins, nucleic acids, cells and tissues, human and plant organs and other biological materials.
Molecular Diagnostic PCR Handbook
PREFACE The Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture is involved in agricultural research and development and assists Member States of FAO and IAEA in improving strategies to ensure food security through the use of nuclear techniques and related biotechnologies, where such techniques have a valuable and often unique role. In particular, molecular diagnostic methods have rapidly evolved in the past twenty years, since the advent of the Polymerase Chain Reaction (PCR). They are used in a wide range of agricultural areas such as, improving soil and water management; producing better crop varieties; diagnosing plant and animal diseases; controlling insect pests and improving food quality and safety. The uses of nucleic acid-directed methods have increased significantly in the past five years and have made important contributions to disease control country programmes for improving national and international trade. These developments include the more routine use of PCR as a diagnostic tool in veterinary diagnostic laboratories. However, there are many problems associated with the transfer and particularly, the application of this technology. These include lack of consideration of: the establishment of quality-assured procedures, the required set-up of the laboratory and the proper training of staff. This can lead to a situation where results are not assured. This book gives a comprehensive account of the practical aspects of PCR and strong consideration is given to ensure its optimal use in a laboratory environment. This includes the setting-up of a PCR laboratory; Good Laboratory Practice and standardised of PCR protocols.
Molecular Biomethods Handbook
Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. Molecular Biomethods Handbook, 2nd Edition expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Part A of the book describes nucleic acid methods, such as gene expression profiling, microarray analysis and quantitative PCR. In Part B, protein and cell-based methods are outlined, in subjects ranging from protein engineering to high throughput screening. Written by a well-established panel of research scientists, Molecular Biomethods Handbook, 2nd Edition provides an up-to-date collection of methods used regularly in the authors’ own research programs.
Molecular analyses : Medical genomics and proteomics
Methods for extraction, manipulation, and characterization of nucleic acids, peptides, and proteins have been rapidly developed. Extraction from a variety of tissues and samples have become routine and automated. Next generation sequencing (NGS) methods have been developed. This book focuses on medically-focused methods
Modern avenues in metal-nucleic acid chemistry
Provides a timely overview of selected relevant developments in a field which previously had a pronounced focus on fundamental chemistry aspects such as coordination principles, structural chemistry, and consequences for biological processes and disease-related medicinal issues. More recently, also as a consequence of the advent of supramolecular chemistry, advancements in nanochemistry, and new bio-analytical methods, novel aspects of this field have emerged. MILS-25 gives a state-of-the-art account on the present state of research in this field, addressing, among others, porous materials, non-covalent interactions, stimuli-responsive bioconjugates, DNA-templated metal arrays and nanoclusters, genotyping, guanine quadruplexes, and riboswitches.
Immobilisation of DNA on Chips II
DNA chips are gaining increasing importance in different fields ranging from medicine to analytical chemistry with applications in the latter in food safety and food quality issues as well as in environmental protection. In the medical field, DNA chips are frequently used in arrays for gene expression studies to identify diseased cells due to over- or under-expression of certain genes, to follow the response of drug treatments, or to grade cancers), for genotyping of individuals, for the detection of single nucleotide polymorphisms, point mutations, and short tandem reports, or moreover for genome and transcriptome analyses in the quasi post-genomic sequencing era. Furthermore, due to some unique properties of DNA molecules, self-assembled layers of DNA are promising candidates in the field of molecular electronics.the main focus of these two volumes is on the immobilization chemistry, considering the various aspects of the immobilization process itself, since different types of nucleic acids, support materials, surface activation chemistries and patterning tools are of key concern.
Immobilisation of DNA on Chips I
the main focus of these two volumes is on the immobilization chemistry, considering the various aspects of the immobilization process itself, since different types of nucleic acids, support materials, surface activation chemistries and patterning tools are of key concern.the focus of the major part of the chapters lies on the coupling chemistry used for DNA immobilization. Successful immobilization techniques for DNA appear to either involve a multi-site attachment of DNA (preferentially by electrochemical and/or physical adsorption) or a single-point attachment of DNA (mainly by surface activation and covalent immobilization or (strept)avidin-biotin linkage).
Heterocyclic Polymethine Dyes : Synthesis, Properties and Applications
Heterocyclic chemistry is the biggest branch of chemistry covering two-thirds of the chemical literature and this book covers the hot topics of frontier research summarized by reputed scientists in the field.
Forces, Growth and Form in Soft Condensed Matter : At the Interface between Physics and Biology
This volume comprises the proceedings of a NATO Advanced Study Institute held at Geilo, Norway, 24 March - 3 April 2003, the seventeenth ASI in a series held every two years since 1971. The objective of this ASI was to identify and discuss areas where synergism between modern physics, soft condensed matter and biology might be most fruitful. The main pedagogical approach was to have lecturers focussing on basic understanding of important aspects of the relative role of the various interaction- electrostatic, hydrophobic, steric, conformational, van der Waals etc. Soft condensed matter and the connection between physics and biology have been the themes of several earlier Geilo Schools. A return to these subjects thus allowed a fresh look and a possibility for defining new directions for research. Examples of soft materials, which were discussed at this ASI, included colloidal dispersions, gels, biopolymers and charged polymer solutions, polyelectrolytes, protein/membrane complexes, nucleic acids and their complexes. Indeed, most forms of condensed matter are soft and these substances are composed of aggregates and macromolecules, with interactions that are too weak and complex to form crystals spontaneously. A characteristic feature is that small external forces, slight perturbations in temperature, pressure or concentration, can all be enough to induce significant structural changes. Thermal fluctuations are almost by definition strong in soft materials and entropy is a predominant determinant of structure, so that disorder, slow dynamics and plastic deformation are the rule. Hence the phrase ‘soft condensed matter’ has been coined.
Enzymatic and Chemical Synthesis of Nucleic Acid Derivatives
The book covers enzymatic (including chemo-enzymatic) methods, with a focus on the synthesis and incorporation of modified nucleosides. The authors also offer a review of innovative approaches for the non-enzymatic chemical synthesis of nucleic acids and their analogs and derivatives, highlighting especially challenging species. The book offers a concise review of the methods that prepare novel and heavily modified polynucleotides in sufficient amount and purity for most clinical and research applications.
DNA manipulation and analysis
Details technologies on recombinant DNA and nucleic acid manipulation that underpin much of the biological sciences and instructions on how to conduct them successfully. Chapters guide the reader through the basics of oligonucleotide synthesis and DNA sequencing; recombinant DNA plasmid work; cell-free experiments and the latest developments in CRISPR approaches to genome modification.
DNA damage detection : Methods and protocols
Explores techniques in DNA damage analysis, important not only for understanding cellular health after exposure to genotoxic agents but also for assessing cellular ability of genome maintenance. The book explores traditional DNA damage measurement methods, as well as modern fluorescence-based analysis and high throughput automatic analysis methods. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
DNA Binders and Related Subjects
advances in technology and the application of new methods to outstanding problems have played a major part in the development of ideas about drug-nucleic acid recognition. The field has undergone an explosive diversification as wider and wider problems became accessible to study using the new ideas and techniques. This volume reflects that diversification by offering accounts of selected areas that illustrate recent advances in the study of ligand–nucleic acid binding over disparate areas of the subject. There are chapters dealing specifically with the invention and application of new methodology, and a particularly thoughtful essay on the interpretation of X-ray diffraction data which may not be as straightforward as is often imagined. Other chapters illustrate the diversity and complexity of drug-DNA binding from several perspectives, referring to particular groups of related compounds or the potential attractions of the less-preferred DNA major groove as a target for nucleotide sequence recognition by ligands.
Computational studies of RNA and DNA
Computational Studies of RNA and DNA includes, in an integrated way, modern computational studies of nucleic acids, ranging from advanced electronic structure quantum chemical calculations through explicit solvent molecular dynamics (MD) simulations up to mesoscopic modelling, with the main focus given to the MD field. It gives an equal emphasis to the leading methods and applications while successes as well as pitfalls of the computational techniques are discussed.The systems and problems studied include: Accurate calculations of base pairing energies / Electronic properties of nucleic acids and electron transfer, through various types of nucleic acid / Calculating DNA elasticity
Low-cost methods for molecular characterization of mutant plants : Tissue desiccation, DNA extraction and mutation discovery : Protocols
Offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds.
Comprehensive biochemistry for dentistry : Textbook for dental students
Combines fundamental concepts of biochemistry and the dental sciences to provide an authentic, coherent and comprehensive text for dental students. It describes in simple language the intricate pathophysiology of biomolecules in health and in diseases of dental and oral tissues. This book also describes the evolution of biochemistry in a chronological order, provides information about the fundamental chemical structure, classification and biological significance of biomolecules, vitamins and hormones, enriched with flow charts and diagrams for easy understanding and quick reference. It includes chapters on nucleic acids, nutrition and serum enzymes and organ function tests, and offers an innovative approach to familiarize dental students with the biochemical composition of enamel, dentine, cementum and saliva, explaining the biochemical basis of dental caries, periodontal diseases, role of fluorides in caries prophylaxis, fluoride toxicity, and the role of amino acids as anti-hypersensitive agents
Chemistry for pharmacy students : General, organic and natural product chemistry ; 2nd ed.
Opens with an overview of the general aspects of chemistry and their importance to modern life, with emphasis on medicinal applications. The text then moves on to discuss the concepts of atomic structure and bonding and the fundamentals of stereochemistry and their significance to pharmacy in relation to drug action and toxicity. Various aspects of organic functional groups, organic reactions, heterocyclic chemistry, nucleic acids and their pharmaceutical importance are then covered in subsequent chapters, with the final chapter dealing with drug discovery and development, and natural product chemistry. Provides a student-friendly introduction to the main areas of chemistry required by pharmacy degree courses. Written at a level suitable for non-chemistry students in pharmacy, but also relevant to those in life sciences, food science, and the health sciences Includes learning objectives at the beginning of each chapter Focuses on the physical properties and actions of drug molecules
