An optimized protocol for mouse bone marrow mesenchymal stromal cells isolation and culture

  • 01 Mar 2022
  • Recently published Research - Pharmacy


Dima Joujeh, Abduljalil Ghrewaty, Chadi Soukkarieh, Adnan Almarrawi, Jamal Abdul Naser Darwicha

Published in

Cellular Therapy and Transplantation (CTT), Volume 10, No. 3-4, October-December 2021.


Mesenchymal stromal cells (MSCs) have stimulated much interest in the scientific community. Mouse MSCs serve as an ideal tool to explore cell biology and therapeutic potential of MSCs. Therefore, establishment of optimal, standardized protocol for mouse MSCs isolation and culture is required. Our aim was to develop and describe an efficient, reliable, and easy-to-perform protocol for isolation and culture of mouse bone marrow mesenchymal stromal cells MSC(M). Our protocol is based on a combination of flushing method and mechanical crushing of the bones. MSC(M) isolated using. Our protocol showed spindle-shaped appearance, positive expression of CD73 and CD44 markers, weak expression of CD34 and CD105, and negative expression for CD11b. They were also able to differentiate into mesodermal lineages such as adipocytes, and osteocytes. We hope that the data presented in this paper are of practical importance and can be used in clinical and research applications, and cell banking.

Keywords: Balb/c mice, bone marrow cells, isolation, mesenchymal stem cells, primary culture.

Link to Read Full Paper