Therapeutic potential of conditioned medium derived from bone marrow mesenchymal stromal cells co-cultured with hepatocytes in alleviation of CCl4-induced liver damage in mice

  • 24 Sep 2022
  • Recently published Research - Pharmacy

Researchers

Dima Joujeh, Abduljalil Ghrewaty, Adnan Almarrawi, Chadi Soukkarieh, Jamal Abdul N. Darwicha

Published in

Cellular Therapy and Transplantation (CTT), Volume 11, No. 2, June 2022.

 


Abstract

Mesenchymal stromal cell-derived conditioned medium therapy is a rapidly developing field of research that seems to have a significant impact on the treatment of various diseases. In the present study, the therapeutic effect of conditioned medium derived from bone marrow mesenchymal stromal cells (BMSCs) co-cultured with hepatocytes (H/BMSCs CM) was investigated for alleviation of CCl4-induced liver damage in mice.

Materials and methods: Hepatocytes were co-cultured with BMSCs for 20 h. H/BMSCs CM were then collected. Mice were intraperitoneally injected with CCl4 twice a week for 7 weeks, followed by injection of H/BMSCs CM. Four weeks after the CM treatment, serum levels of ALT, AST, alkaline phosphatase (ALP), and albumin were determined, liver histopathology was examined, and ELISA assay was performed to measure the serum levels of IL-4 and IFN-γ.

Results: The CM-treated group demonstrated marked decrease in serum levels of ALT and AST compared to RPMI group, without significant differences in the levels of alkaline phosphatase and albumin. Histopathological study demonstrated noticeable improvement in CM treatment group. CM treatment caused an increase in IL-4 production compared to RPMI group, and did not affect the release of IFN-γ.

Our results have shown that H/BMSCs CM treatment attenuates CCl4-induced injury. Further studies should be carried out to elucidate the mechanism of action in order to achieve intended therapeutic effect, which can be translated and optimized.

Keywords: Mesenchymal stromal cells, bone marrow, hepatocytes, co-culture, conditioned medium, liver damage.

Link to Read Full Paper

http://cttjournal.com/en/archive/tom-11-nomer-2/eksperimentalnye-issledovaniya/terapevticheskiy-potentsial-sredy-konditsionirovannoy-stvolovymi-kletkami-kostnogo-mozga-dlya-oslabl/