Author

Haya Ayyal Salman, Amira Suriaty Yaakop, Ahmed Al-Mustafa, Khaled Tarawneh, Saleem Aladaileh, Fuad Al-Rimawi, Fadi Alakhras, Saleh Abu-Lafi, Raghdaa Al Zarzour and Habibah Wahab

Published in

Saudi Journal of Biological Sciences, volume 28, issue 11, Pages 6245-6253, November 2021.

Abstract

Screening of phytochemical Ephedra alte crude extract by GC–MS and HPLC analysis indicated the presence of alkaloids, tannins, flavonoids, terpenoids, and phenolic acid in the extract. The total phenolic content of E. alte methanol extract was 39.43 mg of Gallic acid eq/g, crude E. alte with 56.74, and 2.42 µg Trolox equivalent antioxidant capacity (TEAC)/g of plant extract according to DPPH and FRAP assay, respectively. The antimicrobial activity of E. alte against Staphylococcus aureus, staphylococcus epidermidis, Escherichia coli, and Klebsiellaoxytoca demonstrated a mean zone diameter of inhibition ranging from 0 to 17 mm. The MIC of the extracts ranged from 0.5 to 1.0 mg/mL. E. alte extract inhibits pepsin enzyme activity with IC50 values of 213.67 µg/ml. This study revealed that E. alte extract has pepsin enzyme inhibitory, antibacterial, antioxidant activities. The current outcomes indicate that E. alte might be employed as a natural agent for managing GERD and infectious diseases.

Keywords: Anti-bacterial, Antioxidants, Anti-pepsin, Ephedra alte, GERD, Natural agent.

Link to full paper

https://www.sciencedirect.com/science/article/pii/S1319562X21005544